Review article provides tools for the Rosaceae genomics community Jul 8, 2008
These crops are emerging as attractive organisms for functional genomics studies due to the availability of resources like expressed sequence tags, bacterial artificial chromosome libraries, physical and genetic maps, molecular markers, genetic transformation protocols and bioinformatic tools. In the past few years, for example, researchers at the Virginia Bioinformatics Institute and the Department of Horticulture in the College of Agriculture and Life Sciences at Virginia Tech have developed... (EurekAlert!)
CombiMatrix Launches BAC HD Scan: Diagnostic for Over 125 Genetic Disorders Including Autism Spectrum Disorder Apr 8, 2008
The test utilizes the company's Bacterial Artificial Chromosome (BAC) array technology, and is comprised of 2,437 unique validated loci obtained through the company's partnership with The Center of Applied Genomics (TCAG), in Toronto, Canada ... CombiMatrix Molecular Diagnostics, Inc., a wholly owned subsidiary located in Irvine, California, has developed capabilities of producing arrays that utilize bacterial artificial chromosomes, which also enable genetic analysis. (Primezone Releases)
Identical Twins Not As Identical As Believed Feb 20, 2008
The UAB lab is one of the few worldwide that can make the full genome BAC (bacterial artificial chromosome) arrays that are used to find the changed DNA regions. The findings were published Feb. 14 online in the American Journal of Human Genetics. (Science Daily)
CombiMatrix Molecular Diagnostics Launches Microarray Test for Detection of Autism Spectrum Disorder Dec 18, 2007
Dec. 18, 2007 (PRIME NEWSWIRE) -- CombiMatrix Corporation (NasdaqGM: - ) announced today that its subsidiary, CombiMatrix Molecular Diagnostics (CMDX), has completed the clinical validation of the first of its ATScan(tm) suite of BAC (Bacterial Artificial Chromosome) array CGH (Comparative Genomic Hybridization) based tests. ATScan is designed to detect known genomic copy-number variations (CNVs) associated with Autism Spectrum Disorder (ASD) and this test is now available to physicians and... (Primezone Releases)
Fine-mapping of a QTL influencing pork tenderness on porcine chromosome 2 Oct 13, 2007
Recent advances in porcine genome resources, including high-resolution radiation hybrid and bacterial artificial chromosome (BAC) physical maps, were utilized for development of novel informative markers. Marker density in the ~30-Mb region surrounding the most likely QTL position was increased by addition of eighteen new microsatellite markers, including nine publicly-available and nine novel markers. (BioMed Central)
CombiMatrix Molecular Diagnostics Launches HemeScan MDS for the Management and Diagnosis of Myelodysplastic Syndromes Sep 25, 2007
Sept. 25, 2007 (PRIME NEWSWIRE) -- CombiMatrix Corporation (NasdaqCM: - ) announced today that its subsidiary, CombiMatrix Molecular Diagnostics (or ``CMDX''), has completed the clinical validation of the third of its HemeScan(tm) suite of Bacterial Artificial Chromosome (or ``BAC'') Comparative Genomic Hybridization (or ``CGH'') array-based tests. HemeScan(tm) MDS (myelodysplastic syndromes) is designed to detect clonal prognostic markers in bone marrow aspirates from patients with refractory... (Primezone Releases)
Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region Sep 9, 2007
It is thus essential to understand genetic details of the giant panda MHC. Construction of a bacterial artificial chromosome (BAC) library will provide a new tool for panda genome physical mapping and thus facilitate understanding of panda MHC genes. Results. (BioMed Central)
End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries Sep 7, 2007
We performed large-scale bacterial artificial chromosome (BAC) end-sequencing of two BAC libraries (an EcoRI- and a BamHI-digested library) and conducted an in silico analysis to characterize the obtained sequence data, to make them a useful resource for genomic research on the silkworm (Bombyx mori). Results. (BioMed Central)
Development of a physical map of the soybean pathogen Fusarium virguliforme based on synteny with Fusarium graminearum genomic DNA Aug 3, 2007
Exactly 4,152 Bacterial artificial chromosome (BAC) end sequences from 2,178 large-insert Fusarium virguliforme clones were tested against this sequence. A total of 94 maps of F. graminearum sequence scaffolds, annotated exonic fragments and associated F. virguliforme sequences resulted. (BioMed Central)
News tips from the Journal of Neuroscience Jun 19, 2007
In this weeks Journal, Regan et al. used bacterial artificial chromosome (BAC) transgenes to visualize the expression pattern of two glutamate transporters in mice, glutamateaspartate transporter (GLAST) and glutamate transporter-1 (GLT-1). These two transporters are primarily expressed in astroglia and do the heavy lifting in terms of glutamate uptake in the embryonic and adult brain, respectively. (EurekAlert!)
Horse genome assembled Feb 12, 2007
Approximately 300,000 Bacterial Artificial Chromosome (BAC) end sequences, which provide continuity when assembling a large genome sequence, were contributed to the horse sequencing project by Ottmar Distl, D.V.M., Ph. D. and Tosso Leeb, Ph. (SpiritIndia)
A sequence based synteny map between soybean and Arabidopsis thaliana Jan 9, 2007
An EST, bacterial artificial chromosome (BAC) end sequence and marker amplicon sequence synteny map of soybean and A. thaliana is presented. In addition, all JAVA programs used to create this map are available upon request and on the WEB.. (BioMed Central)
Multiple upstream modules regulate zebrafish myf5 expression Jan 3, 2007
We isolated a 156-kb bacterial artificial chromosome clone that includes an upstream 80-kb region and a downstream 70-kb region of zebrafish myf5 and generated a transgenic line carrying this 156-kb segment fused to a green fluorescent protein (GFP) reporter gene. We find strong GFP expression in the most rostral somite and in the presomitic mesoderm during segmentation stages, similar to endogenous myf5 expression. (BioMed Central)
P[acman] Unlocks Virtually All Areas of the Fruit Fly Genome Dec 6, 2006
Venken first sought a vector that could handle large amounts of DNA efficiently, settling on a bacterial artificial chromosome, which was designed a couple years ago and is known to maintain only a few copies of the DNA (though it can produce a high number of copies if it is induced). Placed into a bacterium, the chromosome integrates into the bacterial vector--a process known as "recombineering." "This methodology is very important because it allows you to very quickly integrate almost any... (Scientific American)
P(acman) Takes A Bite Out Of Deciphering Drosophila DNA Dec 3, 2006
P/phiC31 artificial chromosome for manipulation, or P(acman), combines three recently developed technologies: a specially designed bacterial artificial chromosome (BAC) that allows maintenance of large pieces of DNA in bacteria, recombineering that allows the manipulation of large pieces of DNA that can then be inserted into the genome of the fly at a specific site using phiC31-mediated transgenesis ... The bacterial artificial chromosome, or BAC, he used allows the scientist to maintain large... (Science Daily)
Construction of a 7-fold BAC library and cytogenetic mapping of 10 genes in the giant panda (Ailuropoda melanoleuca) Nov 17, 2006
In order to promote research on this genome, a bacterial artificial chromosome (BAC) library of the giant panda was constructed in this study. Results. (BioMed Central)
NIAS and Sanger Institute Concludes Agreement on Pig Genome Research Aug 15, 2006
NIAS and STAFF will produce a draft sequence of the genome from the Bacterial Artificial Chromosome region commissioned by the Sanger Institute. The result of the joint project is to be announced at the Porcine International Genome Sequencing Consortium. (JCN Network, Japan)
Genomic alterations identified by array comparative genomic hybridization as prognostic markers in tamoxifen-treated estrogen receptor-positive breast cancer Apr 12, 2006
We performed array comparative genomic hybridization (CGH) with 1,440 human bacterial artificial chromosome (BAC) clones to assess copy number changes in 28 fresh-frozen ER-positive breast cancer tissues. All of the patients included had received at least 1 year of tamoxifen treatment. (BioMed Central)
