Job Prospects Drawing Students to Ag Schools Nov 17, 2009
In this photo taken Nov. 11, 2009, University of Illinois senior Tristesse Jones, whose major is crop sciences with a concentration in biological sciences and a minor in chemistry, prepares soybean DNA samples for gel electrophoresis in the Agricultural Science laboratory at the University of Illinois. Jones is one of a growing number of students being drawn to ag schools around the country not by ties to a farm but by science, the job prospects for those who are good at it and, for some, the... (ABC News -- Business)
Protein changes in heart strengthen link between Alzheimer's disease and chronic heart failure Nov 16, 2009
Agnetti points out that the team's protein analysis was only made possible in the last 15 years, and with the development of technologies for detailed chemical analysis, such mass spectrometry and gel electrophoresis. Previously, he says, scientists had mostly focused on genetic changes and their relationship to disease, as opposed to disease-causing alterations to proteins that occur after proteins are made. (EurekAlert!)
KAIKObase: An integrated silkworm genome database and data mining tools Oct 21, 2009
In parallel, proteomic data from two-dimensional polyacrylamide gel electrophoresis in various tissues and developmental stages were compiled into a silkworm proteome database. Finally, a Bombyx trap database was constructed for documenting insertion positions and expression data of transposon insertion lines. (BioMed Central)
Wash hands - keep computers clean Oct 2, 2009
We investigated the association of methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Acinetobacter baumannii, three leading hospital-acquired pathogens, from ward computer keyboards, mice and from clinical isolates in non-outbreak period by pulsed field gel electrophoresis and antibiogram. Results. (BioMed Central)
Parkinson’s Disease: Iron Accumulation To The Point Of Demise Sep 7, 2009
For the first time, they were now able to supply evidence of ferritin in the neuromelanin granules using a combination of diverse techniques (one-dimensional SDS gel electrophoresis, targeted mass spectrometry, western blot analysis, as well as immune transmission electron microscopy). To date, this important iron depot protein had only been proven in glia but not in neurons. (Science Daily)
In vitro antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-beta-lactamase-producing Pseudomonas aeruginosa Aug 10, 2009
Strains used were from different hospitals in Japan and had different pulse-field gel electrophoresis patterns by restriction with SpeI. The minimum inhibitory concentrations of 11 antimicrobial agents (piperacillin, piperacillin/tazobactam, imipenem, meropenem, aztreonam, ceftazidime, amikacin, tobramycin, arbekacin, ciprofloxacin and colistin) were determined using the agar dilution test. The effects of aztreonam, colistin and the combination of aztreonam, ceftazidime and amikacin were... (BioMed Central)
Association of virulence plasmid and antibiotic resistance determinants with chromosomal multilocus genotypes in Mexican Salmonella enterica serovar Typhimurium strains Jul 3, 2009
Multilocus sequence typing (MLST) and macrorestriction fingerprints by pulsed-field gel electrophoresis (PFGE) were used to address the core genetic variation, and genes involved in pathogenesis and antibiotic resistance were selected to evaluate the accessory genome. Results. (BioMed Central)
Novel Cancer Gene And Biomarker Pinpointed Jun 25, 2009
27, 2007) Using two-dimensional gel electrophoresis, 27 differentially expressed proteins were identified in colorectal adenoma, compared with individual-matched normal mucosa and cancer tissue. In those. (Science Daily)
Analyzing DNA by Southern Blot Jun 16, 2009
Using gel electrophoresis the fragments of DNA generated by digestion with a restriction enzyme can be separated according to how big they are ... 1 Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. (Suite101.com)
Aluminum-oxide Nanopore Beats Other Materials For DNA Analysis Jun 6, 2009
(May 18, 2009) Molecular biologists, including the forensic investigators on the popular TV show CSI, use gel electrophoresis to separate DNA fragments from each other in order to analyze the DNA. A team of. . (Science Daily)
Hands-on science at Scituate High School May 21, 2009
Before last week, things like agarose gel electrophoresis, DNA fingerprinting and DNA scissors may have been abstract concepts that Scituate High School biology students had heard about in classroom discussion ... In a series of what the school science department termed in-house field trips, biology students performed agarose gel electrophoresis to distinguish normal red blood cells from cells with sickle cell anemia ... Agarose gel electrophoresis is method that separates DNA fragments by their... (Scituate Mariner, MA)
DNA Gripped In Nanopores: Researchers Analyze Forces On DNA In Gel May 18, 2009
ScienceDaily (May 18, 2009) Molecular biologists, including the forensic investigators on the popular TV show CSI, use gel electrophoresis to separate DNA fragments from each other in order to analyse the DNA. A team of researchers under the leadership of Vici winner Serge Lemay, has now shown for the first time how the gel influences the movement of the DNA. The researchers drove a single DNA molecule through a nanopore in order to analyse the forces on the DNA.. See also. (Science Daily)
What Is Western Blotting? May 10, 2009
In order to most easily detect a specific protein, once it has been size fractionated by polyacrylamide gel electrophoresis, requires getting the protein out of the plastic matrix without losing the resolution that was gained by separating it by size in the first place ... When a protein is separated by gel electrophoresis, and subsequently detected by antibody, it is known as a Western blot. (Suite101.com)
Anatomy of the peanut salmonella crisis Mar 15, 2009
Microbiologist Kara Cooper loads a gel for Pulsed Field Gel Electrophoresis (PFGE), the method used for subtyping or fingerprinting food-borne bacteria, at the Pulse Net lab at the CDC. Over 325,000 such DNA fingerprints exist in a central database. For all the on the peanut crisis and the salmonella outbreak, with an updated list of recalled items, plus background on the scare, go to the AJC's special report. (Atlanta Journal-Constitution)
Why tracking salmonellaoutbreak took months Feb 24, 2009
Some states test every salmonella sample they collect using pulsed-field gel electrophoresis, or PFGE, while others check only some. In Wisconsin and Texas, for instance, only about half are screened. (MSNBC -- Health)
Computer network tracked salmonella outbreak Feb 10, 2009
PulseNet gets its name from a lab technology called pulsed-field gel electrophoresis, which enables investigators to do DNA "fingerprinting" of the infection bacteria and its strain subtype. It's somewhat like an FBI database that can compare fingerprints from an arrest record in California to fingerprints at the scene of a crime in Florida and determine if it's the same crook. (MSNBC -- Technology)
Cattle DNA map leads to quick breakthrough in genetic testing Feb 8, 2009
The test developed by Beever uses low throughput methodology called gel electrophoresis. The DNA material is placed in a gel, and electricity is added to separate the DNA.. (Minnesota Farm Guide, MN)
