Characterization of endemic Shigella boydii strains isolated in Iran by serotyping, antimicrobial resistance, plasmid profile, ribotyping and pulsed-field gel electrophoresis Aug 29, 2008
Ten S. boydii strains isolated from pediatric cases of gastroenteritis and acute diarrhea in Tehran between December 2002 and November 2003 were submitted to serotyping, antimicrobial susceptibility testing, plasmid profile analysis, ribotyping and pulsed field gel electrophoresis (PFGE). Seven isolates were attributed to serotype 2, whereas the remaining three belonged to serotypes 14, 18, 19, respectively. (BioMed Central)
Proteomic analysis identifies MMP-9, DJ-1 and A1BG as overexpressed proteins in pancreatic juice from pancreatic ductal adenocarcinoma patients Aug 16, 2008
To identify novel potential biomarkers for PDAC from pancreatic juice, we carried out difference gel electrophoresis (DIGE) and tandem mass spectrometry (MSMS) to compare the pancreatic juice profiling from 9 PDAC patients and 9 cancer-free controls. Of the identified differently expressed proteins, three up-regulated proteins in pancreatic cancer juice, matrix metalloproteinase-9 (MMP-9), oncogene DJ1 (DJ-1) and alpha-1B-glycoprotein precursor (A1BG), were selected for validation by Western... (BioMed Central)
Anthrax case spurs new germ-gene sleuthing Aug 9, 2008
Kevin N. Roark / LANLAnthrax DNA fluoresces under ultraviolet light in a gel electrophoresis plate. DNA analysis can detect the "fingerprint" of an anthrax strain. (MSNBC -- Environment)
New Biomarker For Early Cancer Detection? Research Reveals That 'MicroRNA' May Fit The Bill Jul 30, 2008
27, 2007) Using two-dimensional gel electrophoresis, 27 differentially expressed proteins were identified in colorectal adenoma, compared with individual-matched normal mucosa and cancer tissue. In those. (Science Daily)
Plant Steroids Offer New Paradigm For How Hormones Work Jul 28, 2008
"In genomics, we aim for a comprehensive survey of all the genes in genome. In proteomics, we're mapping the proteins." Because there can be hundreds of thousands of different proteins in a single organism, proteomics requires techniques, such as 2-dimensional gel electrophoresis, which can process and segregate thousands of proteins at a time based on differences in their size and charge. But even with these methods, isolating the low-abundance signaling proteins was a daunting task. (Science Daily)
A dengue receptor as possible genetic marker of vector competence in Aedes aegypti Jul 15, 2008
(2) The midgut proteins recognized by DENV were also determined by VOPBA after two-dimensional gel electrophoresis. (3) To determine whether the same proteins were identified in all three strains, we obtained polyclonal antibodies against R67 and R64 and tested them against the three strains by immunoblotting; both antibodies recognized the 67 and 64 kDa proteins, corroborating the VOPBA results. (BioMed Central)
Capturing DNA Molecules In A Nanochannel Jun 28, 2008
This presents a range of new options for the separation of fragments (and entire molecules) of DNA. The previous technique, known as gel electrophoresis, involved the use of micro-channels filled with a gel. According to researcher Georgette Salieb-Beugelaar, the laborious and time-consuming process of pouring in the gel can be rendered obsolete by the new method. (Science Daily)
Tainted tomato cases top 750 Jun 27, 2008
A method called pulsed-field gel electrophoresis compares the strain of salmonella from a patient with the outbreak serotype. If they match, it's a confirmed case. (Atlanta Journal-Constitution -- Metro)
Yolk protein is expressed in the insect testis and interacts with sperm Jun 13, 2008
These samples were separated by 2-D gel electrophoresis, and gels were treated with a glycoprotein-detecting probe. We observed a group of abundant glycoproteins in the sample collected after sperm release, which was absent in the sample collected before sperm release. (BioMed Central)
Genetic characterization of clinical and agri-food isolates of multi drug resistant Salmonella enterica serovar Heidelberg from Canada Jun 7, 2008
Chromosomal genetic homogeneity was observed by pulsed-field gel electrophoresis (PFGE), DNA sequence-based typing (SBT) and DNA microarray-based comparative genomic hybridization (CGH). Sixty one of isolates were indistinguishable by PFGE conducted using XbaI and BlnI restriction enzymes. (BioMed Central)
CDC warns of Salmonella risk from dry pet food May 17, 2008
News of the outbreak came to light in June 2007 when PulseNet, an electronic network for sharing molecular data, identified Salmonella enterica serotype Schwarzengrund infections from a handful of states that had indistinguishable pulse-field gel electrophoresis patterns, according to MMWR.. Initial interviews of patients from Pennsylvania who were sickened by the outbreak strain suggested exposure to dogs or dry dog food; 8 (62%) owned one or more dogs, and the other 5 had regular contact with... (CIDRAP)
Teen Helps Design Classroom DNA Experiments Using Common Food Dyes May 15, 2008
ScienceDaily (May 14, 2008) Agarose gel electrophoresis ... One of the most frequently used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic technique often used in genetic or DNA fingerprinting ... Trigiano, R.N., Ownley, B.H., Trigiano, A.N., Coley, J., Gwinn, K.D., Moulton, J.K. Two Simple and Inexpensive Laboratory Exercises for Teaching Agarose Gel Electrophoresis and DNA Fingerprinting. (Science Daily)
DNA fingerprinting simplified May 14, 2008
KNOXVILLE, TN Agarose gel electrophoresis" Most teenagers wouldnt have a clue what this scientific term means, but middle school student Andrew Trigiano knows the protocol inside and out. When Andrew was 12, his father Robert Trigiano, a professor at the University of Tennessee, was looking for an interesting science project for his son. Setting out to compare differences in popular brands of Easter egg dyes, Trigianos project soon grew into a full-blown scientific study and set of... (EurekAlert!)
Performance of a 70-mer oligonucleotide microarray for genotyping of Campylobacter jejuni. May 8, 2008
In the present study we use microarray-based comparative genomic hybridizations (CGH), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to analyze closely related C.jejuni isolates from chicken and human infection. Results. (BioMed Central)
Students dedicate school vacation week to science Mar 8, 2008
Another morning, the students used the techniques of genotyping and gel electrophoresis in a demonstration of how DNA testing can be used for paternity tests and forensic analysis. On the final day of the program, students worked with the roundworm C. elegans to explore its genetics and analyze different mutant traits and how they are inherited. (Cambridge Chronicle, MA)
Genetics of DNA Paternity Testing Mar 4, 2008
After being cut up, the restriction fragments of each genome (mother, father and baby s) are separated using a technique called gel electrophoresis ... Gel electrophoresis enables the lab technician to see which DNA fragments of the mother are shared by the child and, more importantly, if some of the child s DNA fragments are the same size as fragments found in the supposed father s DNA sample. (Suite101.com)
Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS Feb 19, 2008
We analyzed the Escherichia coli proteome response to the exogenous stress of guanidine hydrochloride using 2-dimensional gel electrophoresis and found that RpoS (RNA polymerase sigma factor) was significantly stress responsive. While under the stress condition the total number of soluble proteins decreased by about 7 %, but a 6-fold increase in the level of RpoS was observed, indicating that RpoS is a stress-induced protein. (BioMed Central)
Forensics, fiction, and fingerprints at Sulphur High Feb 12, 2008
Pictured are Sulphur High students using biotechnology equipment on loan from LSU. The students are using gel electrophoresis to make a DNA fingerprint in order to solve a fictitious crime. The students are in G. Broussard's Advanced Biology class. (Sulphur Southwest Daily News, LA)
Little lab of horrors Feb 1, 2008
Then they used polyacrylamide gel electrophoresis to separate out the proteins, and mass spectrometry to identify what type of enzymes the proteins were likely to be. Because some of the enzymes they found were unfamiliar, they searched protein databases to find enzymes with similar structures and noted that some of them were probably not digestive at all. (Nature News Service)
Contaminated Cosmetics Can Be Fatal For Critically Ill Jan 31, 2008
Pulsed-field gel electrophoresis experiments confirmed that all of the strains of B. cepacia bacteria found in patient and environmental samples were from the same bacterial clone. Tests on sealed containers of the moisturizer confirmed that the bacteria had not invaded the product after it had been opened, but that it was contaminated during manufacturing, transportation or storage. (Newsmax)
Mechanism behind viral fusion Jan 24, 2008
The fluorescence and gel electrophoresis experiments revealed a tight self-assembly of TMD in the model membrane. A weak but non-random interaction between TMD and FP in the membrane was found. (BioMed Central)
Characterization of cultured neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb Jan 16, 2008
Therefore, we compared protein expression profiles between NPCs isolated from the subventricular zone and the olfactory bulb using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry. Additionally, it is also unknown whether cultured NPCs isolated from different brain differ in their marker protein expression or growth characteristics. (BioMed Central)
Aboard the biotech bus Jan 10, 2008
Students work through the process of gel electrophoresis, using micropipettes (those CSI turkey basters) to insert hemoglobin into gel blocks that are then electrocuted; students then analyze the results. Students described the lab as very enriching. (Littleton Independent, MA)
Dystrophin deficiency in canine X-linked muscular dystrophy in Japan (CXMDJ) alters myosin heavy chain expression profiles in the diaphragm more markedly than in the tibialis cranialis muscle Jan 9, 2008
In addition, MHC isoforms were detected by gel electrophoresis. Results. (BioMed Central)
Mobile lab brings biotech to town Dec 5, 2007
The students used gel electrophoresis to differentiate between normal hemoglobin and hemoglobin found in individuals with sickle cell disease. "So much in science is shifting from the human body biology to biotechnology," Pierce said. (The Daily Reflector)
Fast Way Of Spotting Multidrug Resistant Bacteria Could Help Stop Outbreaks Dec 1, 2007
Currently all the Royal Free Hospital strains of bacteria are sent to a reference laboratory for typing by a method called pulsed field gel electrophoresis. The new test, called multiplex-PCR, gives results comparable to the ones produced by the reference laboratory. (Science Daily)
Bioclocks work by controlling chromosome coiling Nov 22, 2007
So, the researchers used a standard method, called gel electrophoresis, to measure the extent of a plasmids supercoiling during different points in the day/night cycle. The researchers found a distinct day/night cycle: The plasmid is smaller and more tightly wound during periods of light than they are during periods of darkness. (EurekAlert!)
School likes the results of its lab work Nov 16, 2007
Gel electrophoresis is now more than a multisyllable tongue twister for Hudson Catholic High School students. Thanks to a newly renovated science lab - dedicated and blessed yesterday - Mary Ann Scheiner's forensics class will now be able to perform such a scientific process, which she described as "DNA fingerprinting.". (Hudson Sun, MA)
Teacher diagnosed with MRSA Oct 27, 2007
DNA fingerprinting of MRSA uses pulsed-field gel electrophoresis to determine whether bacteria isolated from different patients are related. This process helps in tracking, tracing and preventing the spread of MRSA.. (New Haven Register, CT)
Story ideas from Molecular & Cellular Proteomics Oct 6, 2007
Fuchu He and colleagues have used a state-of-the-art technique called two-dimensional difference gel electrophoresis to look for proteins whose copies are either increased or decreased in patients with hepatocellular carcinoma ... Article: Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis, by Wei Sun, Baocai Xing, Yi Sun, Xiaojuan Du, Min Lu, Chunyi Hao, Zhuang Lu, Wei Mi, Songfeng Wu, Handong Wei, Xue Gao, Yunping Zhu, Ying Jiang, Xiaohong Qian,... (EurekAlert!)
USDA Defends 18-Day Hold on Beef Recall Oct 5, 2007
Subsequently, USDA scientists conducted a more sophisticated test, known as the Pulsed Field Gel Electrophoresis, that took an additional seven days. After those results became available on Sept. 14, the department then investigated to determine whether the suspected meat could have been contaminated in the consumer's home. (Fox News)
Where Broken DNA Is Repaired Aug 7, 2007
DSB yield can be measured by pulling apart a cell's radiation-severed DNA using gel electrophoresis: the shorter the fragments in the gel bands, the more frequent the breaks ... On the other hand, gel electrophoresis fails to indicate the severity of the damage, or where in the nucleus the double-strand breaks occur ... Most gel electrophoresis studies indicate about 25 to 30 DSBs per gray of gamma rays. (Science Daily)
Pet Turtle Linked to Infant Death Jul 9, 2007
Salmonella isolated from the turtle was indistinguishable by pulsed-field gel electrophoresis to the strain that killed the girl, according to the July 6 issue of Morbidity and Mortality Weekly Report ... Testing with pulsed-field gel electrophoresis revealed they were infected with a Salmonella strain indistinguishable from the one isolated from the patient. (MedPage Today)
First Bacterial Genome Transplantation Changes One Species To Another Jun 30, 2007
As a test of the success of the genome transplantation, the team used two methods 2D gel electrophoresis and protein sequencing, to prove that all the expressed proteins were now the ones coded for by the M. mycoides LC chromosome. Two sets of antibodies that bound specifically to cell surface proteins from each cell were reacted with transplant cells, to demonstrate that the membrane proteins switch to those dictated by the transplanted chromosome not the recipient cell chromosome. (Science Daily)
Artist works wonders with rollers Jun 17, 2007
Limn also has a group show of unusual interest titled "Beneath the Skin." It introduces the appealing work of London painter Klari Reis, who takes the abstract field toward medical and visceral reference, as does Jaq Chartier, who bases her silken pictures on the patterns of gel electrophoresis. The show includes a slightly larger-than-life head by Nathaniel Price densely studded with strike-anywhere matches, their tips facing outward. (San Francisco Chronicle)
Salmonella-tainted peanut butter sickened 628, CDC reports Jun 2, 2007
Pulsed-field gel electrophoresis (PFGE) patterns of Salmonella Tennessee strains isolated from patients were uploaded from state health department databases to CDC databases. Three closely related patterns* were determined to be associated with this outbreak. (Food Consumer)
Salmonella In Peanut Butter Has Infected Over 600 People Jun 2, 2007
On closer examination they noticed that three of the pulsed-field gel electrophoresis (PFGE) patterns of the Salmonella Tennessee strains isolated from many affected patients were closely related, suggesting a common source of contamination. The patients did not come from the same geographical areas, and interviews conducted in November and December 2006 did not reveal any common food exposure. (Medical News Today)
DNA breakage surprisingly rare May 30, 2007
Previous estimates of the number of DSBs came from cruder, indirect tools, which estimated the breakage rate by counting dead cells in a culture, or smashing up the cell and then running a gel electrophoresis. "It's an interesting study, mainly from the technology point of view," , at the UT Southwestern Medical School, who did not participate in the study, told The Scientist. (The Scientist)
SCHOOLS: Science teachers tap in to resources at UofA May 11, 2007
Snell played detective in the Teenage Romance Riddle, using a gel electrophoresis technique to determine if a crime had been committed by a hypothetical girl who may have cheated on her equally hypothetical boyfriend. The kids will love this, said Snell, who intends to order free kits to re-create the crime in her class. (Sierra Vista Herald, AZ)
Restaurants remove tainted beef Apr 22, 2007
Those infected receive a blood test, and also a PFGE, or Pulse Field Gel Electrophoresis. The PFGE uncovers the genetic fingerprint left by the E. coli. (York Daily Record)
Nanoparticles Can Damage DNA, Increase Cancer Risk Apr 19, 2007
She and her team examined the genotoxicity of silica and C60 fullerene nanoparticle suspensions using the alkaline single-cell gel electrophoresis assay (Comet assay) to quantify breaks in single and double stranded DNA. The team chose these particular nanoparticle types because they are commonly used commercially -- in electronics, textiles and sporting goods -- and easy to work with in the laboratory setting. "We observed both dose-dependent and time-dependent increases in DNA damage in breast... (Science Daily)
BioBus makes Center School stop Apr 5, 2007
Information provided by CURE states that the 40-foot, custom-designed mobile laboratory bus and its programs are "designed to generate student interest in, and excitement for, bioscience and to motivate and encourage career exploration in this field. Through the eagerness and attentiveness of the first set of 24 Grade 6 students to venture onto the rolling laboratory, at least in Thomaston, it appears that the BioBus is doing exactly as it has set out to do. All 93 sixth-grade science students,... (Thomaston Express, CT)
Three outbreaks of salmonellosis linked with baby poultry Apr 2, 2007
Laboratory analysis of the isolates by pulsed-field gel electrophoresis (PFGE)* yielded an indistinguishable DNA pattern that was later designated as the outbreak strain. During April--July, the laboratory isolated the outbreak strain from a total of 21 clinical samples obtained from ill persons in Michigan. (Food Consumer)
Regional Science Fair Winners Announced Mar 30, 2007
Third (team): Emily Petry and Gwen Petry of Battlefield for The Effect of Beta Radiation on DNA Analysis via Gel Electrophoresis. Chemistry. (The Burke Times, VA)
Students experiment with high tech biology Mar 24, 2007
Advertisement Students used gel electrophoresis, a technique of using electric current to separate proteins into certain shapes ... That was a wise decision to go the quick route, because some of his students were getting antsy just waiting the 15 minutes for the gel electrophoresis to finish. (Greenwich Time)
Bacteria from patient's dental plaque causes ventilator-associated pneumonia Mar 24, 2007
Protein and DNA profiles of pathogens in plaque and in bronchial alveolar lavage samples were identified using a process called pulsed-field gel electrophoresis. Results showed that genetic profiles of bacteria from tracheal and bronchial samples of the nine patients with pneumonia were identical to profiles of bacteria from their dental plaque. (EurekAlert!)
Mini-med school: a big experience Mar 22, 2007
Day said he would show students a gel electrophoresis procedure, used in DNA testing, similar to operations technicians perform in his molecular biology lab. "We'll show them how we can select colonies to isolate DNA ... and show them some things on the microscopes where we're looking at live cells," Day said. (The Cavalier Daily, VA)
'Microsieve' Device Promises To Speed Up Separation, Sorting Of Proteins Feb 23, 2007
"But the field was using decades-old gel electrophoresis technology. There is a big gap in the need for technology in this area.". Han and Fu therefore devised the anisotropic sieve that is embedded into a silicon chip. (Science Daily)
Tips from the Journals of the American Society for Microbiology Jan 25, 2007
In the study researchers collected plaque samples from twenty children, some with S-ECC and some caries-free (CF), and evaluated the difference in bacterial diversity using denaturing gradient gel electrophoresis (DGGE), a cultivation-free method which isolates total microbial genome DNA. Results showed the S-ECC group exhibited 94. 5 bacterial populations while the CF group exhibited 113. (EurekAlert!)
Rapid, Low-Cost DNA Testing Jan 10, 2007
The most widespread and common method of screening DNA is called gel electrophoresis ... Setting up a lab for gel electrophoresis requires a capital expenditure of 5,000 ... The new process comes after these two steps, saving a final hour of work for the technician, who ordinarily would be doing gel electrophoresis. (Science Daily)
Multidrug-Resistant Salmonella enterica Serotype Typhimurium Associated with Pet Rodents Jan 4, 2007
In August 2004, isolates of Salmonella enterica serotype Typhimurium, which were indistinguishable from one another by pulsed-field gel electrophoresis (PFGE), were obtained from eight hamsters from a Minnesota pet distributor. We conducted an investigation to determine whether human cases of salmonella could be linked to this rodent-borne strain. (New England Journal of Medicine)
