New Electrostatic-based DNA Microarray Technique Could Revolutionize Medical Diagnostics Jul 2, 2008
Often referred to as "gene chips," DNA microarray assays and their RNA counterparts have become one of the most powerful tools for gene-expression profiling, the identification of mutations, and the detection of multiple pathogens in patients afflicted either by multiple diseases or drug-resistant strains of diseases. Aside from their potential future role in personalized medicine, the widespread use of DNA microarray assay devices could have an immediate and profound impact on the treatment of... (Science Daily)
Molecular Science Could Further Improve Leukemia Survival, Say St. Jude Researchers Mar 21, 2008
One is gene-expression profiling, which measures the amount of messenger RNA made by different genes in different cell types; and the other is pharmacogenetics. RNA is the decoded form of genes and is a signal that those genes have been active. (PR Newswire)
Immune Cells Fighting Chronic Infections Become Progressively 'Exhausted,' Ineffective Oct 22, 2007
Using a technique called gene-expression profiling, Wherry and his colleagues identified 490 genes whose activity in T cells is altered during a chronic viral infection. Closer study at different time points using a 22-gene subset of the larger group of genes provided molecular signatures of progressive T-cell exhaustion. (Science Daily)
How phytol suppresses arthritis May 10, 2007
The aim of this study was to use gene-expression profiling to understand the protective effect against arthritis of activation of NADPH oxidase in the immune system ... Hence, global gene-expression profiling on inguinal lymph nodes was performed 10 days after the induction of pristane-induced arthritis (PIA) and phytol administration. (BioMed Central)
Models of Breast Cancer Feb 11, 2007
In fact, gene-expression profiling can be used to predict which tumour is likely to be metastatic. Some have even proposed that tissue-specific profiles can predict the site of metastasis. (Suite101.com)
Concordance among Gene-Expression–Based Predictors for Breast Cancer Aug 10, 2006
Background Gene-expression profiling studies of primary breast tumors performed by different laboratories have resulted in the identification of a number of distinct prognostic profiles, or gene sets, with little overlap in terms of gene identity. Methods To compare the predictions derived from these gene sets for individual samples, we obtained a single data set of 295 samples and applied five gene-expression based models: intrinsic subtypes, 70-gene profile, wound response, recurrence score,... (New England Journal of Medicine)
Genetic Parallels Found Between Lung Development And Lung Cancer Jul 11, 2006
More importantly, they show that gene-expression profiling can predict a lung cancer's prognosis, and that cancers whose gene expression pattern resembles gene expression during the earliest stages of lung development have the worst prognosis of all. "This confirms our earlier finding of the importance of normal organ development in understanding cancer," says Isaac Kohane, MD, PhD, director of the CHIP program and a co-author on the paper. (Science Daily)
Burkitt’s Lymphoma — The Message from Microarrays Jun 8, 2006
The two studies differ in many important ways, but both reach the same conclusion: the gene-expression profiling of cases classified as Burkitt's lymphoma by expert pathologists identifies a characteristic genetic signature that clearly distinguishes this tumor from cases of diffuse large-B-cell lymphoma. Furthermore, the microarray method seems to outperform the expert pathologists: 17 percent1 and 34 percent2 of cases with the gene-expression. (New England Journal of Medicine)
A Biologic Definition of Burkitt’s Lymphoma from Transcriptional and Genomic Profiling Jun 8, 2006
Methods We performed gene-expression profiling using Affymetrix U133A GeneChips with RNA from 220 mature aggressive B-cell lymphomas, including a core group of 8 Burkitt's lymphomas that met all World Health Organization (WHO) criteria. A molecular signature for Burkitt's lymphoma was generated, and chromosomal abnormalities were detected with interphase fluorescence in situ hybridization and array-based comparative genomic hybridization. (New England Journal of Medicine)
Molecular Diagnosis of Burkitt’s Lymphoma Jun 8, 2006
We examined whether gene-expression profiling could reliably distinguish Burkitt's lymphoma from diffuse large-B-cell lymphoma ... Conclusions Gene-expression profiling is an accurate, quantitative method for distinguishing Burkitt's lymphoma from diffuse large-B-cell lymphoma. (New England Journal of Medicine)
