Novel Polymer Delivers Genetic Medicine, Allows Tracking Oct 8, 2009
This image shows a deconvoluted micrograph of a HeLa cell transfected with DNA-polymer beacon complexes. The DNA is labeled with fluorescein isothiocyanate and appears green in the image. (Science Daily)
New stably transfected bioluminescent cells expressing FLAG epitope-tagged estrogen receptors to study their chromatin recruitment Sep 9, 2009
We describe here the generation of such cells, using an estrogen receptor negative HELN cell line that was derived from HeLa cells stably transfected with an ERE-driven luciferase plasmid ... In MCF-7 cells, the recruitment of ERalpha also exhibited a biphasic behaviour; with the second peak however not so important than in the HeLa cell lines ... However, the relative importance of recruitment between 1 hr and 4 hr was found to be different in HeLa cell line expressing exogenous tagged ERalpha... (BioMed Central)
Hitting target genes in drug discovery Feb 19, 2009
As an alternative technology, we applied highly efficient gene-targeting with recombinant adeno-associated virus to precisely integrate a luciferase reporter gene into exon 1 of the HeLa cell tumor necrosis factor-alpha (TNF-a) gene. Drugs known to induce TNF-a expression were then used to compare the authenticity of gene-targeted and randomly integrated transcriptional reporters. (BioMed Central)
