What is touch DNA? Aug 9, 2008
Here s how it works: Investigators recover cells from the scene, then use a process called polymerase chain reaction (PCR) to make lots of copies of the genes. Next, scientists mix in fluorescent compounds that attach themselves to 13 specific locations on the DNA and give a highly specific genetic portrait of that person. (Scientific American)
HHV-6A in Syncytial Giant-Cell Hepatitis Aug 7, 2008
At the onset of the disease, the detection of HHV-6A (but not HHV-6B) DNA in plasma, in affected liver tissue, and in single micromanipulated syncytial giant cells with the use of two different polymerase-chain-reaction (PCR) assays indicated the presence of active HHV-6A infection in the patient. Expression of the HHV-6A specific early protein, p41/38, but not of the HHV-6B specific late protein, p101, was demonstrated only in liver syncytial giant cells in the absence of other infectious... (New England Journal of Medicine)
Dinucleotide repeat polymorphism in Fms-like tyrosine kinase-1 (Flt-1) gene is not associated with preeclampsia Jul 17, 2008
The region containing the dinucleotide repeat polymorphism of the Flt-1 gene was amplified by polymerase chain reaction (PCR) from the DNA samples and was analyzed by direct PCR sequencing. Results. (BioMed Central)
Agency to Unveil TB Test Jul 1, 2008
The new diagnostic test -- called a line-probe assay -- is made by Germany's Hain Lifescience GmbH, and uses a technology known as polymerase chain reaction, or PCR, to identify genes linked with resistance to two first-line TB drugs, rifampin and isoniazid. The information will enable doctors to more quickly start treatment, quelling the bug's spread. (Wall Street Journal)
Monsoon illness claims 1 more life Jun 23, 2008
"We will be testing his blood samples with a high-tech PCR test to check if it was indeed leptospirosis," she said. Another 137 Mumbaikars were hospitalised with fever. (Times of India)
Genetic and biochemical studies in Argentinean patients with variegate porphyria Jun 21, 2008
We have studied at molecular level 18 new Argentinean patients biochemically diagnosed as VP. PPOX gene was amplified in one or in twelve PCR reactions ... For RT-PCR studies RNA was retrotranscripted, amplified and sequenced. (BioMed Central)
Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM Jun 20, 2008
We designed the competitive multiplex PCR to identify the carrier with exonal deletions ... There was one family identified with whole exons deletion, and the carriers were successfully recognized by multiplex PCR. By HRM, the different melting curve patterns were easily identified in 25 out of 28 cases (89%) and 15 out of 15 (100%) carriers. (BioMed Central)
A New Arenavirus in Transplantation Jun 12, 2008
Second, diagnostic tests for LCMV (polymerase chain reaction, serologic tests, or viral isolation) would undoubtedly have detected the agent, had these tests been used on initial screening. The omission of these tests. (New England Journal of Medicine)
Acinetobacter baumannii in Intensive Care Unit: a Novel System to Study Clonal Relationship Among the Isolates. Jun 9, 2008
The Repetitive sequence-based PCR (REP-PCR) has been recognized as an effective method and it was recently adapted to an automated format known as the DiversiLab system. Methods. (BioMed Central)
Third Wave buys Stratagene patents May 31, 2008
Third Wave said the new chemistry also opens new research markets to the company, including the $500-million quantitative polymerase chain reaction market. In a press release, Third Wave president and CEO Kevin Conroy said: "The next-generation Invader chemistries strengthened by this acquisition will give Third Wave's clinical products fundamental competitive advantages in the most valuable molecular diagnostic markets.". (Milwaukee Business Journal, WI)
Med BioGene Appoints Diagnostics Industry Veteran Dr. Heiner Dreismann to Board of Directors May 29, 2008
While at Roche, Dr. Dreismann led the introduction in Europe of the company's PCR (polymerase chain reaction) and, now industry-standard, real-time PCR-based tests. PCR is a Nobel-prize winning technology which has been recognized as one of the most important scientific developments of our generation ... PCR allows scientists to copy a single segment of DNA billions of times, making it possible to take a specimen, copy its genetic sequence, and, within hours, generate a test sample sufficient to... (CCNMatthews Press Releases)
US Senate to consider extra $400m for NIH May 26, 2008
Reagents (protein, genomic, PCR, cell-culture). Separation Sciences (chromatography). (LabTechnologist.com)
A novel de novo mutation in the serine-threonine kinase STK11 gene in a Korean patient with Peutz-Jeghers syndrome May 22, 2008
Using DNA samples obtained from the patient and his family members, we sequenced nine exons and flanking intron regions of the STK11 gene using polymerase chain reaction (PCR) and direct sequencing. Results. (BioMed Central)
The evolution of teen science May 20, 2008
The highly sophisticated work using mouse-cell cultures and polymerase chain reaction (PCR) will probably see light in a peer-reviewed academic journal. The gold has left Kirkland Lake, but millions of tonnes of tailings from the mines haven't. (Toronto Star)
AECL pulls plug on reactors after millions spent May 17, 2008
In the past three years alone, AECL spent more than $200-million as it sought an answer to a vexing problem known as a positive power coefficient of reactivity (PCR). The reactor is supposed to have a negative coefficient of reactivity, meaning the nuclear reaction would slow down if the power in the core increased. (Globe and Mail)
Teen Helps Design Classroom DNA Experiments Using Common Food Dyes May 15, 2008
Explaining another experiment from the study, Trigiano continued: "The second laboratory exercise requires more extensive equipment and a more advanced set of skills, but the exercise has been completed successfully by middle school-level through graduate-level students and teachers. In this exercise, the internally transcribed spacer region of the ribosomal subunit for a fungus, plant, and insect are amplified and separated electrophoretically on agarose gels. A simple crime is solved using... (Science Daily)
Kids More Vulnerable to Swimming-Related Illness May 9, 2008
The newer method called quantitative polymerase chain reaction (QPCR) may be able to reveal contamination levels in as little as two hours. To determine how well testing methods were able to gauge illness risk, the researchers tested water quality. (MEDLINEplus)
Lab In A Drop: Pocket-sized PCR Rapid Test Including Sample Preparation May 8, 2008
They have now developed a rapid test for genetic diagnosis that combines the preparation of biological samples with a polymerase chain reaction (PCR) on one chip ... PCR allows gene sequences to be duplicated and identified to identify a disease trigger, for example ... Because of the slow heating and cooling processes, laboratory PCR usually takes several hours. (Science Daily)
Services crippled as doctors at LNJP continue to strike May 8, 2008
Their other demands included lodging an FIR against the family and deployment of a PCR van in the hospital. "They (family of the deceased) alleged the patient died due to medical negligence, which is not true. We need security for ourselves," he added. (Zee News)
Bureaucracy Hampers an E. Coli Weapon May 7, 2008
GeneSystems, which has raised $15 million in venture capital, accomplishes this feat by doing away with traditional slow-growing Petri dish cultures, instead harnessing a molecular biology technique called polymerase chain reaction (PCR) that produces as many as 100 billion copies of a strand of DNA in a single afternoon. With a large sample to work from, lab technicians can more easily spot bacteria such as E. coli using diagnostic equipment. (BusinessWeek)
Cancer Could Return Unless Stored Ovarian Tissue Undergoes Adequate Testing Before Re-implantation May 2, 2008
" Dr Meirow, who leads the fertility preservation programme in the IVF Unit at Chaim Sheba Medical Center, Tel Hashomer (headed by Professor Jehoshua Dor), carried out the research with Professor Dina Ben Yehuda, director of the Hematology Division at Hadassah University Hospital, Jerusalem. Dr Meirow said that fertility centres with close connections to cancer and haematological centres should be able to work together in order to adopt the correct methods for checking stored tissue. Before... (Science Daily)
Anti-fibrotic Mechanism Of A Chinese Medicinal Herb May Inspire Drug Development May 2, 2008
Using human hepatic stellate cells and a real-time quantitative PCR method, this research was performed by physicians from the Beijing Genomics Institute, the Liver Research Center of the Beijing Friendship Hospital, and the Institute of Medicinal Plant Development of the Chinese Academy of Medical Sciences, China. Further research should be done to explain the mechanism for Cpd 861's regulation of collagen-related gene expression and to identify the active antifibrotic ingredient in Cpd 861. (Science Daily)
Stored Ovarian Tissue Needs Testing before Re-Implantation May 2, 2008
Only one test ever detected minimal residual disease in ovarian tissue: modern, highly sensitive, real-time polymerase chain reaction (PCR), a test that amplifies sections of DNA to detect molecular markers that would indicate the presence of cancer cells. The authors suggested that, as methods for detecting cancer cells are improving all the time, and ovarian tissue can be stored for more than 10 years, tests to detect residual disease should be carried out just before transplantation rather... (MEDLINEplus)
GREEN GODDESS Apr 22, 2008
" Hudson helped choose the ingredients for Babaii for WildAid's Hydrating Shampoo and Hydrating Conditioner, like volcanic ash from the Vanuatu Islands in the South Pacific, which is said to add volume to the hair, to butter harvested from the Amazon, used for its hydrating properties. (Proceeds benefit WildAid, an eco-force working towards ending the illegal wildlife trade and save endangered species around the world.) Kiehl's introduced their antioxidant-rich Superbly Restorative body lotion... (New York Post -- Entertainment)
New Technologies Offer More Accurate Means Of Diagnosis And Monitoring Cancer Apr 21, 2008
Using a laboratory technique called methylation-specific polymerase chain reaction, which enables the production of millions of copies of a specific DNA sequence in a short period of time, the researchers analyzed promoter methylation (a DNA modification that shuts down gene expression) of the tumor suppressor genes p16 and FHIT in 1,774 samples of oral and bronchial tissue taken from the study participants. They found that methylation patterns observed in the oral tissues correlated with those... (Science Daily)
Expression Profiling with RNA from Formalin-Fixed, Paraffin-Embedded Material Apr 20, 2008
g. by quantitative reverse transcriptase PCR, QPCR) remain a major challenge ... The RNA is suitable for expression measurement by QPCR when used in combination with an optimized cDNA synthesis protocol and TaqMan assays specific for short amplicons. (BioMed Central)
Herceptin(R) Helps Eradicate HER2-Positive Breast Tumours When Given Before Surgery Apr 18, 2008
" The GeparQuattro results are consistent with other Herceptin neoadjuvant studies, such as NOAH (NeOAdjuvant Herceptin, including 228 evaluable HER2-positive patients)(2) and TECHNO (Taxol-Epirubicin-Cyclophosphamid-Herceptin Neoadjuvant), reinforcing the strong evidence for the benefits associated with Herceptin in the early stages of HER2-positive breast cancer. "It is very reassuring to see that the GeparQuattro study confirmed the significant benefits observed with Herceptin in other... (Canada Newswire)
Herceptin and chemo improves response rates without major adverse effects in HER2 breast cancer Apr 18, 2008
3% of women with HER2-positive tumours who had received both chemotherapy and trastuzumab; whereas the pathologic complete response rate (pCR, complete disappearance of cancer cells from the breast) was 19 ... Prof Untch said: Previous studies have shown that HER2-positive women who do not receive trastuzumab never have a pCR rate that exceeds 20 ... Our study has shown that it is possible to combine trastuzumab with anthracycline/taxane chemotherapy regimen without any significant adverse... (EurekAlert!)
Have your genetic code unravelled? Apr 17, 2008
Instead, the sample is directly amplified by a process called polymerase chain reaction. The long string of "rungs" is then broken up and unravelled by parallel sequencers, and the data then fed into a powerful computer for reassembly and analysis. (iAfrica.com)
Minor raped by three cops in Ludhiana Apr 12, 2008
According to an FIR registered in the civil lines police station, she was overpowered by three policemen deployed there on PCR duty and taken to a corner in the park. They forcibly took away the boy accompanying her, gagged him and allegedly raped the girl, the report said. (India Times, India)
With annual deaths from malaria on the rise: Scientists ask 'where is all the money going?' Apr 12, 2008
Bed nets work at night and have helped contain the spread of malaria, but what about the hours when people arent in their beds" The protection during the day is minimal with current insecticides that cannot be used on a regular basis. The search for a vaccine is necessary, but so are the immediate needs of at-risk communities. The scientists say that organizations such as the Bill da Gates Foundation and The World Health Organizations Roll Back Malaria, lead in funding research for drug and... (EurekAlert!)
Norovirus strikes in Lake County Apr 11, 2008
O'Connor said the three cases were confirmed via Polymerase Chain Reaction testing. People who do get sick remain contagious for anywhere from 2 to 16 days after the symptoms go away. (Missoulian, MT)
Preliminary Data From Pitt County Memorial Hospital Study Show Benefit of Active Surveillance on Reducing MRSA VAP Rates in Surgical ICU Apr 7, 2008
During the 12-month intervention period, MRSA VAP rates in the SICU decreased by 68 percent due to the implementation of a comprehensive infection control program that included hospital-wide (universal) active surveillance using the BD GeneOhm(TM) MRSA real-time polymerase chain reaction (PCR) diagnostic test. The analysis of the SICU-specific data shows that the MRSA VAP rate decreased from 1. (PR Newswire)
HPV, Periodontitis Work In Tandem To Increase Risk Of Tongue Cancer Apr 7, 2008
" The study involved 30 patients newly diagnosed with squamous cell carcinoma on the base of the tongue between 1999 and 2005 at Roswell Park Cancer Institute for whom data was available on both periodontitis and tumor HPV status. Cumulative history of periodontitis was determined by assessing the loss of alveolar bone, the bones that underlie and support the teeth, via X-ray. Tumor status was identified from paraffin-embedded tumor samples analyzed by polymerase chain reaction. Analysis... (Science Daily)
MRSA in hospital intensive care -- what's growing where? Mar 31, 2008
Our technique allows us to characterise the genes from micro-organisms using a gene amplification technique called polymerase chain reaction (PCR). This lets us expand tiny samples enough to identify individual strains of bacteria, and to spot particular antibiotic resistance genes, says Gemma Kay. (EurekAlert!)
BARACLUDE data show low resistance over 5 years in nucleoside-naive hepatitis B patients Mar 25, 2008
1 Virologic breakthrough is defined as a greater than or equal to 1 log increase in HBV DNA from nadir, as measured by the polymerase chain reaction or PCR assay. 2 Undetectable viral load is defined as HBV DNA levels less than 300 copies/mL, as measured by PCR assay. (EurekAlert!)
Biosensing nanodevice to revolutionize health screenings Mar 25, 2008
Moreover, Frasch says, Studies with the F1-ATPase in my laboratory show that since it can detect single DNA molecules, it far exceeds the detection limits of conventional PCR [polymerase chain reaction] technology. . (EurekAlert!)
BD GeneOhm(TM) MRSA Assay Part of Largest U.S. Study Using Universal Active Surveillance to Significantly Reduce MRSA Infection Rates Mar 18, 2008
FRANKLIN LAKES, N.J., March 17 /PRNewswire-FirstCall/ -- BD Diagnostics, a segment of BD (Becton, Dickinson and Company) (NYSE: ), today announced that a new study, "Universal Surveillance for Methicillin-Resistant Staphylococcus aureus in 3 Affiliated Hospitals," published in Annals of Internal Medicine, demonstrates that universal surveillance of all hospital admissions for methicillin-resistant Staphylococcus aureus (MRSA) using the BD GeneOhm(TM) MRSA real-time polymerase chain reaction... (PR Newswire)
Handheld DNA Detector Mar 17, 2008
24, 2008) Researchers in Hong Kong have miniaturized technology needed to perform the versatile polymerase chain reaction (PCR) -- widely used in criminal investigations, disease diagnosis, and a range of. (Oct. (Science Daily)
Damaged Veins Heal Faster With Heparin Treatment, Laboratory Study Finds Mar 15, 2008
Using real-time polymerase chain reaction tests, the researchers were also able to look at patterns of gene expression in harvested veins in culture. As with the cell staining, they saw signs that the pre-treated mice reacted in a way that suggested fastest recovery. (Science Daily)
Genetic Markers Found For Lung Cancers Most Likely To Recur Mar 15, 2008
Under licensing agreement between the Johns Hopkins University and OncoMethylome, methylation specific PCR was licensed to OncoMethylome and they are entitled to a share of the royalties received by the University from sales of the licensed technology. Brock receives research support from OncoMethylome Sciences. (Science Daily)
Bio-Sensor Quickly Detects Anthrax, Smallpox And Other Pathogens Mar 8, 2008
Other available sensors are based on immunoassays or PCR (polymerase chain reaction), which take much longer and/or are not as sensitive as CANARY.. Rider and colleagues first reported the success of CANARY (which stands for Cellular Analysis and Notification of Antigen Risks and Yields) in the journal Science in 2003. (Science Daily)
The New Age of Molecular Diagnostics for Microbial Agents Mar 6, 2008
1 Currently, the majority of surveillance and discovery efforts use methods based on sequences of known agents namely, competitive polymerase chain reaction (PCR) and microarrays. Such efforts fail, however, when the agents in. (New England Journal of Medicine)
Toxins In Oil Spills And Cigarette Smoke Prevent Stem Cells From Becoming Cartilage Mar 5, 2008
In the current study, polymerase chain reaction (PCR), a technique that measures gene expression levels, revealed the genetic changes caused by exposure to BaP in mouse stem cells. Among the many factors that influence gene expression are transcription factors, proteins designed to direct genes to create more or less of a protein. (Science Daily)
Warnex Offers New Prostate Cancer Screening Service Mar 5, 2008
The Warnex PCA3 screening service for prostate cancer uses advanced PCR technology to detect mRNA from the prostate cancer gene 3 (PCA3). Warnex is the first laboratory to offer this service in Canada. (CCNMatthews Press Releases)
Roles of TNF-|alpha| gene polymorphisms in the occurrence and progress of SARS-Cov infection:a case-control study Mar 1, 2008
PCR sequencing based typing (PCR-SBT) method was used to determine the polymorphisms of TNF-alpha gene in locus of the promoter region and univariate logistic analysis was conducted in analyzing the collected data. Results. (BioMed Central)
Research Specialist - Molecular Biology and Mouse ES Cells Feb 29, 2008
The duties include performing ES cell targeting for gene targeting/transgenic construction; screening targeting/transgenic ES cell colonies by PCR; expanding positive ES cell clones and preparing ES cells for aggregation. The research specialist will harvest embryonic fibroblast cells, prepare feeders for ES cell culture and will derive new mouse ES cell lines. (Nature News Service)
Urine-Based Test for Prostate Cancer Promising Feb 12, 2008
Using a technique called quantitative polymerase chain reaction, the researchers measured the expression of seven putative prostate cancer biomarkers, including PCA3, in sedimented urine from 234 men having prostate biopsy or radical prostatectomy (removal of the prostate). Among this cohort, biopsy results confirmed a diagnosis of prostate cancer in 138 patients, while 96 patients were cancer-free. (MEDLINEplus)
Children's Hospital scientists identify possible target for prevention and treatment of pneumonia Feb 12, 2008
Additional research interests of Dr. Kolls include gene therapy, lung immunology, lung host defenses, tumor necrosis factor, pneumocytis carinii pneumonia, ethanol, gene expression, polymerase chain reaction and molecular biology. . (EurekAlert!)
Multiple strand displacement amplification of mitochondrial DNA from clinical samples Feb 8, 2008
Further, body fluid samples useful for cancer early detection are often difficult to amplify with traditional PCR methods ... DNA was isolated from clinical samples and sent to NIST. Samples were amplified by PCR and those with no visible amplification were re-amplified using the Multiple Displacement Amplificaiton technique of whole genome amplification ... All samples were analyzed by mitochip for mitochondrial DNA sequence to compare sequence concordance of the WGA samples with respect to... (BioMed Central)
Scientists confirm new virus responsible for deaths of transplant recipients in Australia Feb 7, 2008
After failing to implicate an agent using other methods including culture, PCR and viral microarrays, RNA from the transplanted liver and kidneys was analyzed using rapid sequencing technology established by 454 Life Sciences and bioinformatics algorithms developed at Columbia ... "The small pieces of viral genetic material recovered through this powerful high throughput sequencing method were used to design specific tests for detecting the virus in clinical samples and enabling detailed... (EurekAlert!)
Diagnosis Of Cutaneous Leishmaniasis Improved With New Techniques Jan 29, 2008
Although QT-NASBA was highly sensitive, another technique, the so-called real-time Reverse Transcriptase PCR was found to be more suitable due to its greater time efficiency and lower costs. In practice, neither of these two techniques will be quickly deployed in developing countries. (Science Daily)
Unions, Dems Wrangle Over Nevada • • • • • • • • Company In The News:• Genoptix Focuses On Tests For Cancers Of The Blood Jan 29, 2008
And lab work being as sophisticated as it is these days, that could mean tests by a lot of different methods: flow cytometry, chromosome analysis, polymerase chain reaction and so on. The hem/onc needs to gather up these results and sort out a diagnosis. (Investors Business Daily)
Aveda Expands its Green Efforts at New York Fashion Week Fall/Winter 2008 Jan 27, 2008
"(2) Over 20% of carbon dioxide greenhouse gas emissions worldwide are due to forest loss (The Food and Agriculture Organization of the United Nations, December 2005). Paper must be a minimum of 10% post-consumer recycled (PCR) Source: Aveda Email Story Set News Alert Print Story Sponsor Results Free $50,000 practice account with real-time charts, news and research.www.forex.comFree Forex Demo & Training. Trade 24 hrs-a-day 5.5 days a week.www.GFTforex.comFree 3-bureau Credit Report includes... (Yahoo! Wire -- Entertainment News)
Scientists Create First Synthetic Bacterial Genome -- Largest Chemically Defined Structure Synthesized In The Lab Jan 25, 2008
They did so using short, single strands of synthetically produced, commercially available DNA (known as oligonucleotides) and using an adaptation of polymerase chain reaction (PCR), known as polymerase cycle assembly (PCA), to build the phi X genome. The team produced the synthetic phi X in just 14 days. (Science Daily)
Breakthrough technologies for 2008 and beyond Jan 24, 2008
The company's Verigene system leverages nanotechnology to move genetic and protein testing to the point of care, thereby overcoming the time--and cost-intensive limitations of conventional polymerase chain reaction tests. Photogallery. (Globe and Mail)
Elderly couple manhandled Jan 22, 2008
By the time we reached, a PCR jeep had also arrived there ... As many as seven PCR jeeps were called in and the two were arrested, said Mr. Garg, a resident of Civil Lines. (Hindu)
American Chemical Society's Weekly PressPac -- Jan. 16, 2008 Jan 22, 2008
Wishing your Valentine good heart health on February 14 and throughout 2008" Then consider the food some people love to hate, and hand over a gift bag of broccoli along with that heart-shaped box of chocolates. Researchers in Connecticut are reporting impressive new evidence that eating broccoli may protect against heart disease. Their study is scheduled for the Jan. 23 issue of ACS Journal of Agricultural and Food Chemistry, a bi-weekly publication. Researchers have known for years that... (EurekAlert!)
Economical Way To Boost Vitamin A Content Of Corn Found Jan 19, 2008
Polymerase chain reaction (PCR) allowed them to amplify and sequence the different versions (alleles) of the genes of interest, to find the alleles that boosted levels of vitamin A precursors in the plant. This approach led to an important discovery. (Science Daily)
New Method Developed To Identify Genetic Determinants Of Alzheimer's Disease Jan 17, 2008
This assay combines PCR and DHPLC and can be used to conduct efficient genotyping of the human population, which in turn will help in the diagnosis and treatment of Alzheimer's disease ... The polymerase chain reaction (PCR) was designed to generate the 191-bp amplicons containing two common polymorphisms within codons 112 and 158 in exon4 of the APOE gene ... The PCR amplicons for each sample were subjected to denaturing high-performance liquid chromatography (DHPLC) analysis, which was... (Science Daily)
Fateful Friday 15 years ago Jan 15, 2008
"On the Harrison conviction, Ivey has a second PCR (post-conviction relief petition) currently pending in the state Supreme Court. On the Montgomery case, Ivey currently has a Habeas plea pending in Federal District Court," Plowden said. "The 12/28 execution date has subsequently been stayed by both the state Supreme Court and the federal court.". (Orangeburg Times and Democrat, SC)
Delhi Police gets 18 hoax calls a day Jan 4, 2008
A total of 6,370 bogus calls were made to the Police Control Room (PCR) last year, keeping the sleuths on their toes ... "Any call to the PCR cannot be ignored like that and someone has to rush to the spot. Some did it for fun, others to create trouble for their neighbours or so," a senior police official said ... Last year, a whopping 11,55,247 calls were made to the PCR as against 9,95,691 in 2006. (India Times, India)
Gargling Could Help Detect Cancer Jan 3, 2008
" But he said a panel of markers chosen from a more extensive initial set of genes is already showing better results in research now being conducted by his group. The method is simple -- a patient brushes the surfaces of the oral cavity with an exfoliating brush, then gargles and rinses with 20 milliliters of normal saline. A specialized form of polymerase chain reaction allows the detection of hypermethylated tumor suppressor genes in the salivary rinse. Hypermethylation prevents transcription... (MedPage Today)
Whatever happened to... Dec 22, 2007
Hugh Rienhoff, a trained clinical geneticist and bioentrepreneur had bought himself a PCR machine and a gel-box so that he could sequence in hopes of uncovering the disorder that has left her weak and unable to put on much weight. She turned four earlier this month, and Rienhoff says a friend is now helping with additional studies on her DNA as well as sequencing genes from him and his wife. (Nature News Service)
Four hurt as car rams into police barricade Dec 17, 2007
Soon Station House Officer S. J. Kaur accompanied by several policemen reached the spot and the injured were taken to the trauma centre in two PCR vans. Later in the day, several senior police officers visited the hospital to inquire about the condition of the injured policemen. (Hindu)
