Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection Oct 11, 2007
In addition, expressions of HSP27, 60, and Prx-2 in GCA specimens were further confirmed by immunohistochemical and western blot analyses. Conclusions. (BioMed Central)
Differential regulation of iron chelator-induced IL-8 synthesis via MAP kinase and NF-kappaB in immortalized and malignant oral keratinocytes Sep 13, 2007
Phosphorylation and degradation of the I-kappa B were analyized by Western blot. Results. (BioMed Central)
OUR OPINION: State board makes right call on Senate proposal Sep 6, 2007
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Summer-school scientists Aug 18, 2007
On a recent afternoon he showed a visitor the fluorescent microscope and Western blot machine he had learned to use from his mentor, research fellow Kulandayan Subramanian. "When I first came to the lab, I was freaking out -- I saw all these biohazard signs and I was scared to touch anything," he said. (Boston Globe)
Former UPenn postdoc faked images Aug 8, 2007
The ORI investigation, published in the Federal Register on July 16, concluded that Roovers had faked 19 panels of Western blot data, which appeared on 11 figures in 3 studies published between 2001 and 2003, Dahlberg said ... The editors "found various pieces of Western blots cut and pasted either within a figure or between figures," said Birnbaum, who said. (The Scientist)
Bernese Mountain Dogs Prone To Infection By Lyme Disease-related Bacteria Jul 16, 2007
The team used an enzyme-linked immunosorbent assay (ELISA) as well as a Western blot test to see whether the dogs had developed antibodies against B. burgdorferi. Of the Bernese Mountain Dogs, 58 percent had developed antibodies against B. burgdorferi, compared with only 15 percent of the control dogs. (Science Daily)
Bernese Mountain Dogs susceptible to spirochete infection Jul 13, 2007
Seroprevalence of B. burgdorferi was assessed by ELISA and Western blot and was 58% in Bernese Mountain Dogs compared to 15% in control dogs. This difference was significant. (BioMed Central)
National HIV Testing Day --- June 27, 2007 Jun 27, 2007
Patients who had positive rapid tests were given risk-reduction counseling and asked to provide a whole blood or oral specimen for confirmatory testing by Western blot. Testing procedures for the three sites differed by the location within the ED where HIV testing was offered and by the personnel responsible for testing and counseling. (Food Consumer)
Adult stem cell figure retracted Jun 14, 2007
In March, the magazine reported that two Western blot figures within a 2001 study in Blood journal resembled each other, despite the fact that they were describing different proteins. that the blots were similar to a blot used to describe yet a third protein type in a submitted by Verfaillie and others. (Scientist)
The 2007 Life Sciences Industry Awards Apr 18, 2007
The sales representatives "come into the lab and sell not just an instrument but a suite of products." James Smyth, a researcher at the University of California, San Francisco, uses Bio-Rad's VersaDoc MP 4000 image analysis system for DNA gel imaging and Western blotting analysis. "We can do multiplexed Western blotting," says Smyth ... "This one can do chemiluminescence for Western blot," she says, with additional features such as the ability to image fluorescent and colorimetric samples. (The Scientist)
Autoimmune Hearing Loss Mar 31, 2007
Sensorineural Hearing Loss in Autoimmune Disease. In 1979, the physician McCabe first described a group of patients with sudden sensorineural hearing loss (related to the inner ear) who improved after being treated with corticosteroids. (Suite101.com)
Identification and characterization of bovine regulator of telomere length elongation helicase gene (RTEL): molecular cloning, expression distribution, splice variants and DNA methylation profile Mar 6, 2007
Four splice variants were identified within the transcripts in both the coding and 5-untranslated regions; Western blot revealed that the most abundant splice variant SV-1 was translated to a truncated isoform of RTEL. The different 5'UTRs imply alternative transcription start sites in the promoter; Bovine RTEL was transcribed at the blastocyst stage, and expression levels were highest in adult testis, liver and ovary. DNA methylation analysis of tissues that differed significantly in expression... (BioMed Central)
A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila Mar 1, 2007
The functional expression of these genes was demonstrated by western blot analysis with MSP1 specific antibodies and by a blasticidin growing assay. Conclusions. (BioMed Central)
Degradation of the LDL receptors by PCSK9 is not mediated by a secreted protein acted upon by PCSK9 extracellularly Mar 1, 2007
Incubation of a secreted, truncated LDLR without cytoplasmic and transmembrane domains, as well as membrane fractions from HepG2 cells, with conditioned medium containing PCSK9, did not reduce the amount of LDLR as determined by western blot analysis. Thus, the LDLR is not degraded by PCSK9 on the cell surface. (BioMed Central)
Nicotine: The Link Between Cigarette Smoking And Kidney Disease? Jan 31, 2007
Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7) ... Blots were incubated overnight with one of nine antibodies, which included mouse anti- 2-nAChR mAb s, anti- 3-nAChR mAb s, or anti- 4-nAChR mAb s. Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. (Science Daily)
Nicotine: The link between cigarette smoking and kidney disease progression? Jan 30, 2007
Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7) ... Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. (EurekAlert!)
Lack of Association between Antimyelin Antibodies and Progression to Multiple Sclerosis Jan 25, 2007
Antibodies were assessed by Western blot analysis at baseline, and the results compared with the time and rate of progression to clinically definite multiple sclerosis or a diagnosis of multiple sclerosis as defined by an international panel (the McDonald criteria) ... Conclusions Serum antibodies against MOG and MBP, as detected by Western blot analysis, are not associated with an increased risk of progression to clinically definite multiple sclerosis in patients who have had a clinically... (New England Journal of Medicine)
Discovering biomarkers from gene expression data for predicting cancer subgroups usingneural networks and relational fuzzy clustering Jan 6, 2007
Our findings suggest the possibility of developing specialized microarray chips or use of real-time qPCR assays or antibody based methods such as ELISA and western blot analysis for an easy and low cost diagnosis of the subgroups. 1999-2007 BioMed Central Ltd unless otherwise stated. (BioMed Central)
'A shocking lack of evidence' Oct 26, 2006
Both Montagnier and Colizzi have seen the results of a western blot, a test to detect proteins: they are "indeterminate", says Montagnier. "They say nothing," adds Colizzi. (Nature News Service)
FDA CAPSULES Oct 17, 2006
NEW HIV TEST. The FDA has approved the Aptima HIV-1 RNA Qualitative Assay, the first test for detection of genetic material in HIV. The Aptima test can be used as an alternative to the Western blot, an existing test used to confirm positive results in HIV-screening tests. www. (Newsday -- Health)
Nicotine Receptors On Kidneys May Speed Kidney Damage Oct 11, 2006
They found nicotine increased the production of fibronectin by about 50 percent as measured by western blot analysis, a laboratory procedure that detects proteins in a given sample of tissue, then measures protein levels with a specialized light meter. Cells with no nicotine had 36 relative light units (RLU), while cells exposed to nicotine had 51 RLU.. (Science Daily)
FDA Approves Test To Help Diagnose Main Virus That Causes AIDS Oct 6, 2006
This test has important implications for medical diagnostic use because it could be a potential alternative to the traditional Western blot test now used for confirmation of HIV-1 infection when screening tests for HIV-1 antibodies are positive. In addition, the Western blot can, in some instances, be difficult to interpret and may not always provide a conclusive result. (U.S. Food and Drug Administration)
An infectious agent of deception, exposed through proteomics Sep 29, 2006
A standard assay called Western blot confirmed the abundance increases of that small group of proteins during infection. . (EurekAlert!)
CDC Recommends Broad HIV Screening Sep 22, 2006
If it is positive, a more specific test, called a western blot, is performed ... The lower figure is for a negative ELISA, the higher for a positive test that would require a western blot and more extensive post-test counseling. (The Ledger)
Genome-wide gene expression profiling of human mast cells stimulated by IgE or Fc{epsilon}RI-aggregation reveals a complex network of genes involved in inflammatory responses Aug 16, 2006
Our data was validated using Real-Time-PCR; ELISA and western blot. We confirmed that IgE alone does not trigger mast cell-immediate responses, such as calcium signals, degranulation or protein-phosphorylation. (BioMed Central)
Lyme disease: Facing Loudoun's silent epidemic Aug 3, 2006
"Western blot tests can produce false negatives," said Dr. Leila Zackrison, a Fairfax-based Lyme specialist and rheumatologist. "It still misses people; it's just a big mess.". (Centreville Times, VA)
Form of palsy gains recognition Jul 28, 2006
Dear Dr. Donohue: Will you enlighten me on my husband's latest diagnosis -- progressive supranuclear palsy (Herald-Tribune)
Slowing Alzheimer's disease by keeping mind and body active Jul 24, 2006
In accordance with this, a significant enhancement of microglial activity was found by Western blot and morphometric analyses of microglia, which often surround and infiltrate A; plaques. In addition, activating cellular protein degradation pathways provides another means of removing excess A. (EurekAlert!)
Protein Tied To Usher Syndrome May Be Hearing's 'Missing Link' Jun 28, 2006
Additional experiments using western blot analysis, a technique that identifies individual proteins in a substance by separating them from one another by mass and testing how they react to certain antibodies, demonstrated that the antibody that recognizes protocadherin-15 in mice also binds to the TLA.. The team also analyzed the amino acid sequences of protocadherin-15 and discovered four distinct forms three of which are present in various developmental stages of the mouse inner ear. (Science Daily)
The Netherlands reports second human case of mad cow disease Jun 23, 2006
A study published in 1996 indicated that a Western blot analysis of infecting prions obtained from 10 variant CJD patients and BSE-infected animals had similar molecular characteristics that were distinct from prions obtained from patients with other types of CJD (Nature 1996;383:685-90). An experimental study involving inoculation of a panel of inbred mice with the agents causing BSE and variant CJD substantially increased the strength of the scientific evidence for a causal association between... (Food Consumer)
Long-term RNAi can be deadly May 26, 2006
Enzyme-linked immunosorbent assay (ELISA)-based screening and Western blot analysis found no evidence of. Another theory was that the shRNAs were toxic because they acted on non-target RNAs, "but we had similar lethal results with six different targets and 23 different shRNAs, making off-targeting unlikely as the cause," Kay said. (The Scientist)
Sleuthing Lyme May 1, 2006
SF Chronicle Submissions. More than 25,000 people in the United States contracted Lyme disease in 2005, but the actual number of cases, the Centers for Disease Control and Prevention concedes, may be 10 times higher than that. (San Francisco Chronicle -- Opinion)
Cloning and expression analysis of two distinct hypoxia-inducible factor alpha isoforms, gcHIF-1alpha and gcHIF-4alpha, from the hypoxia-tolerant grass carp Apr 20, 2006
Northern blot analysis showed that mRNA levels of gcHIF-1alpha and gcHIF-4alpha differ substantially under normoxic and hypoxic conditions, while Western blot studies demonstrated that the endogenous protein levels for both gcHIF-1alpha and gcHIF-4alpha are similarly responsive to hypoxia. Our findings suggest that both gcHIF-1alpha and gcHIF-4alpha are differentially regulated at the transcriptional and translational levels. (BioMed Central)
Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties Apr 11, 2006
NF-kappa-B activation was measured by western blot and immunocytochemistry and cytokine secretion was detected by a protein array. Results: This study shows that S. pneumoniae isolates from pneumonia patients (n=298) can vary by as much as 1000-fold in their ability to bind to human lung epithelial cells. (BioMed Central)
Alabama Cow With Mad Cow Born Before Feed Ban - USDA Mar 20, 2006
Officials confirmed mad cow in the animal on Monday, using the so-called Western blot test. A second confirmatory test that takes more time to complete, called the immunohistochemistry, also came back positive on Wednesday. (Planet Ark, United States)
Electric stimulation enhances chemotherapeutic efficacy Mar 18, 2006
The relationship between MDR1 expression and the intracellular accumulation of doxorubicin as well as the cellular distribution of MDR1 was investigated by computerized image analysis immunohistochemistry and Western blot techniques ... Immunohistochemical, Western blot and fluorescence analysis revealed that AC not only decreases MDR1 expression but also changes its cellular distribution from the plasma membrane to the cytosol. (BioMed Central)
USDAwill dig up infected cow Mar 16, 2006
First, two initial "rapid" screening tests were run, followed by a test called Western blot. The third, called immunohistochemistry, or IHC, was finished Wednesday. (MSNBC -- Health)
R-CALF: Sample From Alabama Santa Gertrudis Positive For BSE Mar 15, 2006
The U.S. Department of Agriculture (USDA) on Monday announced that the National Veterinary Services Laboratory in Ames, Iowa, confirmed through a Western blot test that a Santa Gertrudis cow in Alabama tested positive for bovine spongiform encephalopathy (BSE). USDA officials said a dentition examination by a local veterinarian indicated the animal likely was at least 10 years old or more, meaning she was born before implementation of the 1997 feed ban. (CattleNetwork.com)
Third U.S. BSE Case Won't Affect Talks on Beef Trade, Health Ministry Says Mar 14, 2006
The USDA laboratory in Ames, Iowa, confirmed the disease using a Western Blot test, John Clifford, the department's chief veterinary officer, said yesterday. Meat from the animal didn't enter the human or animal food chains, he said. (Bloomberg -- Japan)
Mad Cow Disease Confirmed In Alabama Mar 14, 2006
Two versions of the initial "rapid" screening test suggested the cow had the disease, and a more detailed Western blot confirmed that finding ... Two versions of the initial "rapid" screening test suggested the cow had the disease, and a more detailed Western blot confirmed that finding. (CBS News)
US Confirms 3rd Case of Mad Cow in 27 Months Mar 14, 2006
He said the new case was confirmed through the Western Blot test, which was added to USDA's battery of tests last year. But Clifford insisted that the discovery of a new case of mad cow disease in the United States should not scare other nations away from American beef. (Planet Ark, United States)
Third US case of mad cow confirmed in Alabama cow Mar 14, 2006
A more accurate Western blot test at the National Veterinary Services Laboratory in Ames, Iowa, proved positive for BSE, said Dr. John Clifford, the U.S. Agriculture Department's top veterinarian. "The animal was buried on the farm and did not enter the animal or human food chains," Clifford told a teleconference with reporters. (Middle East North Africa Financial Network)
