NK-like homeodomain proteins activate NOTCH3-signaling in leukemic T-cells Oct 19, 2009
Proteins were analyzed by western blot, immuno-cytology and immuno-precipitation. For overexpression studies cell lines were transduced by lentiviruses. (BioMed Central)
Parkinson’s Disease: Iron Accumulation To The Point Of Demise Sep 7, 2009
For the first time, they were now able to supply evidence of ferritin in the neuromelanin granules using a combination of diverse techniques (one-dimensional SDS gel electrophoresis, targeted mass spectrometry, western blot analysis, as well as immune transmission electron microscopy). To date, this important iron depot protein had only been proven in glia but not in neurons. (Science Daily)
Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma Sep 6, 2009
The expression of Cripto-1 at mRNA level was detected by the reverse transcription-polymerase chain reaction (RT-PCR) and real time RT-PCR, and western blot was used to examine the protein expression ... The results of real-time RT-PCR and western blot showed that the expression level of Cripto-1 was markedly higher in NPC cell lines than that in the immortalized nasopharyngeal epithelial cell at both mRNA and protein levels. (BioMed Central)
Predicting sorafenib sensitivity Aug 24, 2009
Expression levels of pERK were determined by immunocytochemical quantification together with western blot analysis, and pERK density values were also calculated. Correlation analyses were then carried out between the IC50 values of drugs and pERK density values. (BioMed Central)
Selenoprotein N is dynamically expressed during mouse development and detected early in muscle precursors Aug 23, 2009
Using qRT-PCR, Western blot and whole mount in situ hybridization, we characterized in detail the spatio-temporal expression pattern of the murine Sepn1 gene during development, focusing particularly on skeletal muscles. Results. (BioMed Central)
Acute effect of electroacupuncture at the Zusanli acupoints on decreasing insulin resistance as shown by lowering plasma free fatty acid levels in steroid-background male rats Aug 2, 2009
In addition to an insulin challenge test (ICT) and IVGTT, the plasma levels of FFAs were measured and western blot was performed to help determine the effects of EA on the insulin resistant state. Results. (BioMed Central)
Citrus auraptene suppresses cyclin D1 and significantly delays N-methyl nitrosourea induced mammary carcinogenesis in female Sprague-Dawley rats Jul 30, 2009
The concentration of auraptene in rat tissues was analyzed by reverse phase HPLC. Cyclin D1 expression in MCF-7 cells and rat tumors was measured by western blot ... In comparison, western blot analysis of rat mammary tumors (n=10 per group) confirmed that auraptene (500 ppm) significantly reduced (p<0. (BioMed Central)
Psychotherapy Of Depression Changes Biological Parameters? Jul 7, 2009
pCREB in T lymphocytes was determined by Western blot; the BDNF plasma levels with solid-phase ELISA. Psychopathology was evaluated with the Hamilton Rating Scale for Depression (HAMD). Thirty patients meeting DSM-IV criteria for major depressive episodes (MDE) were recruited into this 6-week study. (Science Daily)
Gene Expression in Lungs of Mice Lacking the 5-hydroxytryptamine Transporter Gene May 10, 2009
Representative genes were confirmed by quantitative RT-PCR and western blot. Results. (BioMed Central)
What Is Western Blotting? May 10, 2009
What Is Western Blotting ... What Is Western Blotting ... What Is Western Blotting. (Suite101.com)
An iron-based beverage, HydroFerrate Fluid (MRN-100), alleviates oxidative stress in murine lymphocytes in vitro May 3, 2009
The effect of MRN-100 on Bcl-2 and Bax protein levels was determined by Western blot. Results: Results show, as expected, that culture of splenic cells with H2O2 alone results in a significant increase in cell death (apoptosis) as compared to control (CM) cells. (Nutrition Journal)
Aryl hydrocarbon receptor pathway activation enhances gastric cancer cell invasiveness likely through a c-Jun-dependent induction of matrix metalloproteinase-9 Apr 17, 2009
RT-PCR and western blot analysis showed that both CYP1A1 mRNA and protein expression were increased in a dose-dependent manner following TCDD treatment and AhR antagonist resveratrol (RSV) could reverse this TCDD-induced CYP1A1 expression. To determine whether TCDD treatment of AGS cells results in an induction of MMP-9 expression, we detected MMP-9 mRNA using RT-PCR and detected MMP-9 enzymatic activity using gelatin zymography. (BioMed Central)
Distinct gene subsets in pterygia formation and recurrence: dissecting complex biological phenomenon using genome wide expression data Mar 10, 2009
Interesting molecules were further investigated with immunohistochemistry, Western blots, and comparison with tear proteins from pterygium patients ... Western blot shows increased protein expression of beta-microseminoprotein, a protein up-regulated in human cutaneous squamous cell carcinoma. (BioMed Central)
Id2 promotes the invasive growth of MCF-7 and SKOV-3 cells by a novel mechanism independent of dimerization to basic helix-loop-helix factors Mar 4, 2009
Protein levels of Id2 and its mutants and E-cadherin were determined by western blot analysis and mRNA levels of Id2 and its mutants were determined by RT-PCR. The effects of Id2 and its mutants on cell proliferation were determined by [3H]-thymidine incorporation assay and the 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) dye method. The in vitro invasion potential of cells was evaluated by Transwell assay. (BioMed Central)
Carestream Molecular Imaging Releases Western Blot Kits For Added Convenience, Better Performance Feb 23, 2009
Carestream Molecular Imaging today released convenient, cost-effective kits for performing western blots -- a popular analytical technique used to detect proteins ... KODAK X-SIGHT Western Kits provide industry-leading components and step-by-step protocols designed to deliver peak performance from X-SIGHT Nanosphere Conjugates in western blots ... "The X-SIGHT Nanosphere Conjugates, when used with the components supplied in the Western Kit, outperformed our current western blot method," said... (Yahoo News -- Press Releases)
Arrested neural and advanced mesenchymal differentiation of glioblastoma cells- comparative study with neural progenitors Feb 15, 2009
Methods Following methods were used to compare glioblastoma cells and GFAP+NNP (NHA): exposure to neural differentiation medium, exposure to adipogenic and osteogenic medium, western blot analysis, immunocytochemistry, single cell assay, BrdU incorporation assay. To characterize glioblastoma cells EGFR amplification analysis, LOH/MSI analysis, and P53 nucleotide sequence analysis were performed. (BioMed Central)
HIV & AIDS Feb 7, 2009
ELISA and Western blot tests For years, the only available test for HIV was the enzyme-linked immunosorbent assay (ELISA) test that looked for antibodies to the virus in a sample of your blood ... If the repeat test was also positive for HIV antibodies, you'd then have another confirming blood test called the Western blot test, which checks for the presence of HIV proteins ... The Western blot test was important because you may have non-HIV antibodies that cause a false-positive result on the... (CNN -- Health)
