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    News and Articles on Western blot



    Native human autoantibodies targeting GIPC1 identify differential expression in malignant tumors of the breast and ovary  Aug 25, 2008
    The current study employs cELISA, flow cytometry, Western blot analysis as well as immunocytochemistry, and immunohistochemistry. Data is analyzed statistically with the Fisher one-tail and two-tail tests for two independent samples. (BioMed Central)

    A human monoclonal autoantibody to breast cancer identifies the PDZ domain containing protein GIPC1 as a novel breast cancer-associated antigen  Aug 25, 2008
    The current study employs immunocytochemistry, immunohistochemistry, Western blot analysis as well as Northern blots, Scatchard binding studies and finally SEREX analysis for target antigen identification. Results. (BioMed Central)

    Proteomic analysis identifies MMP-9, DJ-1 and A1BG as overexpressed proteins in pancreatic juice from pancreatic ductal adenocarcinoma patients  Aug 16, 2008
    Of the identified differently expressed proteins, three up-regulated proteins in pancreatic cancer juice, matrix metalloproteinase-9 (MMP-9), oncogene DJ1 (DJ-1) and alpha-1B-glycoprotein precursor (A1BG), were selected for validation by Western blot and immunohistochemistry ... Increased MMP-9, DJ-1 and A1BG expression in cancerous pancreatic juice were confirmed by Western blot. (BioMed Central)

    Summary for Patients  Aug 5, 2008
    These tests included a serum enzyme-linked immunoassay, a single serum Western blot test, CD4 count, and plasma HIV-1 RNA measurement. Results of the confirmatory tests were not available the day of the emergency department visit. (Annals of Internal Medicine)

    Connexin-43 upregulation promotes tumor cell adhesion  Jul 23, 2008
    High-resolution immunofluorescence microscopy and Western blot analysis was performed using a connexin-43 monoclonal antibody. Calcein Orange Red AM dye transfer by fluorescence imaging was used to evaluate the gap junction function. (BioMed Central)

    Incorrect Diagnosis of Lyme Disease  Jul 21, 2008
    Positive results on both the ELISA test and Western Blot test are required by the Center for Disease Control (CDC) in order to report a case of Lyme disease ... The Western Blot test is far more accurate because it looks for proteins shed from the bacteria and the blebs ... IDSA guidelines restrict physicians from ordering the Western Blot test before the ELISA test or if the ELISA test results are negative. (Suite101.com)

    Clinical study shows biological and clinical activity in relapsed leukemia patients  Jun 5, 2008
    Caspases -8 and -9 were activated in the CLL cells from all six evaluable patients, as measured by western blot analysis. DNA fragmentation in patients' CLL cells also was observed, strengthening the evidence that GCS-100 induces apoptosis. (EurekAlert!)

    'PLoS ONE' STUDY:  A Low Dose of Dietary Resveratrol Partially Mimics Caloric Restriction and Retards Aging Parameters in Mice  Jun 4, 2008
    GLUT4 protein abundance was determined in soleus and EDL muscles by western blotting (n = 16 muscles per group) ... (A) SIRT1 levels in liver, skeletal muscle and brain (n = at least four animals per group) were determined by Western blot analysis ... GLUT4 protein abundance was determined in soleus and EDL muscles by western blotting (n = 16 muscles per group). (USA Today -- Tech)

    Expression of TRPC6 channels in human epithelial breast cancer cells  May 2, 2008
    Molecular (Western blot and RT-PCR), and immunohistochemical techniques were used to investigate TRPC6 expression. To investigate the channel activity in both MCF-7 cells and hBCE we used electrophysiological technique (whole cell patch clamp configuration). (BioMed Central)

    Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice  Apr 3, 2008
    Surprisingly, using the same injection and dosing regimen, we found no significant difference in dystrophin expression by western blot between the NG-PEI2K-PEG550, CG-PEI2K-PEG5K, and non-functionalized PEI2K-PEG550 copolymers. Dose-response experiments using the CG-PEI2K-PEG5K copolymer with total ESO ranging from 3-60 micrograms yielded a maximum of about 15% dystrophin expression. (BioMed Central)

    Neurologic Benefit of Lyme Therapy Short-Lived  Mar 27, 2008
    Fallon, from Columbia University in New York, and colleagues investigated the benefits of 10 weeks of intravenous ceftriaxone (trade name Rocephin) in patients with previously treated Lyme disease who continued to show memory impairments and tested positive for Lyme disease using a Western blot. A total of 37 patients were randomly assigned to receive intravenous ceftriaxone or placebo (saline solution) for 10 weeks. (MEDLINEplus)

    Aminopeptidase N (APN)/CD13 inhibitor, Ubenimex, enhances radiation sensitivity in human cervical cancer  Mar 19, 2008
    Molecular changes of apoptosis were determined by Western blot. Apoptosis was evaluated by Annexin-V PI staining (flow cytometry analysis) and the Tunel method. (BioMed Central)

    Autism's origins: Mother's antibody production may affect fetal brain  Feb 26, 2008
    To test their hypothesis, the research team used a technique called immunoblotting (or Western blot technology), in which antibodies derived from blood samples are exposed to adult and fetal brain tissue to check whether the antibodies recognize and react against specific brain proteins. Comparing the antibody-brain interaction in samples obtained from 100 mothers of autistic children and 100 mothers of children without autism, researchers found either stronger reactivity or more areas of... (EurekAlert!)

    Berberine enhances inhibition of glioma tumor cell migration and invasiveness mediated by arsenic trioxide  Feb 25, 2008
    Zymography and Western blot analyses provided information on the effect of As2O3 and berberine on the intracellular translocation and activation of protein kinase C (PKC), and some PKC-related downstream factors. Most assays were performed three times, independently, and data were analyzed using ANOVA.. (BioMed Central)

    Fenretinide-induced apoptosis of Huh-7 hepatocellular carcinoma is retinoic acid receptor beta dependent  Jan 1, 2008
    Apoptosis was determined by caspase-3 cleavage with western blot, DNA double-strand breaks with TUNEL assay, and phosphatidylserine translocation with flow cytometry analysis. Gene expression of nuclear receptors was assessed by real-time PCR. Transactivation assay and chromatin immunoprecipitation (ChIP) were conducted to evaluate the activation of retinoid X receptor alpha (RXRalpha)/retinoic acid receptor beta (RARbeta) pathway by fenretinide. (BioMed Central)

    Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma  Jan 1, 2008
    Morphology observation, Annexin V-FITC/PI staining, cell cycle analysis and western blot were used to further elucidate the cytotoxic mechanism of TGA. Implanted mouse H22 hepatoma model was used to demonstrate the tumor growth inhibitory activity of TGA in vivo. Results. (BioMed Central)

    HIV testing: accrediting laboratories  Dec 7, 2007
    Patients are also advised to confirm their positive status through a Western Blot test. But for all the efforts to get themselves audited by TNSACS, the private laboratories stand to gain much. (Hindu)

    Overtaken By Hype  Dec 1, 2007
    In 1987, no one wanted to hear about AIDS. Twenty years later, the wheel has come full circle. The spokes, however, are completely different. (India Times, India)

    Stem cells model disease  Nov 15, 2007
    Using RT-PCR and Western blot analysis, they found that the undifferentiated stem cells normally transcribed the Fragile X gene and produced the FMR protein. But when the cells were induced to differentiate, both the FMR1 mRNA and FMR protein levels dropped significantly, suggesting that differentiation is necessary to trigger the silencing mutation and cause the symptoms of the disease. (The Scientist)

    Cognitive Impairment Due To Chronic Lyme Disease Can Be Treated  Oct 13, 2007
    All patients had to meet criteria for memory impairment at the start of the study and they were also required to have a positive IgG Western blot for Lyme disease at study entry. Key findings from the Neurology paper are as follows. (Science Daily)

    Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection  Oct 11, 2007
    In addition, expressions of HSP27, 60, and Prx-2 in GCA specimens were further confirmed by immunohistochemical and western blot analyses. Conclusions. (BioMed Central)

    Differential regulation of iron chelator-induced IL-8 synthesis via MAP kinase and NF-kappaB in immortalized and malignant oral keratinocytes  Sep 13, 2007
    Phosphorylation and degradation of the I-kappa B were analyized by Western blot. Results. (BioMed Central)

    OUR OPINION: State board makes right call on Senate proposal  Sep 6, 2007
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    Summer-school scientists  Aug 18, 2007
    On a recent afternoon he showed a visitor the fluorescent microscope and Western blot machine he had learned to use from his mentor, research fellow Kulandayan Subramanian. "When I first came to the lab, I was freaking out -- I saw all these biohazard signs and I was scared to touch anything," he said. (Boston Globe)

    Former UPenn postdoc faked images  Aug 8, 2007
    The ORI investigation, published in the Federal Register on July 16, concluded that Roovers had faked 19 panels of Western blot data, which appeared on 11 figures in 3 studies published between 2001 and 2003, Dahlberg said ... The editors "found various pieces of Western blots cut and pasted either within a figure or between figures," said Birnbaum, who said. (The Scientist)

    Bernese Mountain Dogs Prone To Infection By Lyme Disease-related Bacteria  Jul 16, 2007
    The team used an enzyme-linked immunosorbent assay (ELISA) as well as a Western blot test to see whether the dogs had developed antibodies against B. burgdorferi. Of the Bernese Mountain Dogs, 58 percent had developed antibodies against B. burgdorferi, compared with only 15 percent of the control dogs. (Science Daily)

    Bernese Mountain Dogs susceptible to spirochete infection  Jul 13, 2007
    Seroprevalence of B. burgdorferi was assessed by ELISA and Western blot and was 58% in Bernese Mountain Dogs compared to 15% in control dogs. This difference was significant. (BioMed Central)

    National HIV Testing Day --- June 27, 2007  Jun 27, 2007
    Patients who had positive rapid tests were given risk-reduction counseling and asked to provide a whole blood or oral specimen for confirmatory testing by Western blot. Testing procedures for the three sites differed by the location within the ED where HIV testing was offered and by the personnel responsible for testing and counseling. (Food Consumer)

    Adult stem cell figure retracted  Jun 14, 2007
    In March, the magazine reported that two Western blot figures within a 2001 study in Blood journal resembled each other, despite the fact that they were describing different proteins. that the blots were similar to a blot used to describe yet a third protein type in a submitted by Verfaillie and others. (Scientist)

    The 2007 Life Sciences Industry Awards  Apr 18, 2007
    The sales representatives "come into the lab and sell not just an instrument but a suite of products." James Smyth, a researcher at the University of California, San Francisco, uses Bio-Rad's VersaDoc MP 4000 image analysis system for DNA gel imaging and Western blotting analysis. "We can do multiplexed Western blotting," says Smyth ... "This one can do chemiluminescence for Western blot," she says, with additional features such as the ability to image fluorescent and colorimetric samples. (The Scientist)

    Autoimmune Hearing Loss  Mar 31, 2007
    Sensorineural Hearing Loss in Autoimmune Disease. In 1979, the physician McCabe first described a group of patients with sudden sensorineural hearing loss (related to the inner ear) who improved after being treated with corticosteroids. (Suite101.com)

    Identification and characterization of bovine regulator of telomere length elongation helicase gene (RTEL): molecular cloning, expression distribution, splice variants and DNA methylation profile  Mar 6, 2007
    Four splice variants were identified within the transcripts in both the coding and 5-untranslated regions; Western blot revealed that the most abundant splice variant SV-1 was translated to a truncated isoform of RTEL. The different 5'UTRs imply alternative transcription start sites in the promoter; Bovine RTEL was transcribed at the blastocyst stage, and expression levels were highest in adult testis, liver and ovary. DNA methylation analysis of tissues that differed significantly in expression... (BioMed Central)

    A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila  Mar 1, 2007
    The functional expression of these genes was demonstrated by western blot analysis with MSP1 specific antibodies and by a blasticidin growing assay. Conclusions. (BioMed Central)

    Degradation of the LDL receptors by PCSK9 is not mediated by a secreted protein acted upon by PCSK9 extracellularly  Mar 1, 2007
    Incubation of a secreted, truncated LDLR without cytoplasmic and transmembrane domains, as well as membrane fractions from HepG2 cells, with conditioned medium containing PCSK9, did not reduce the amount of LDLR as determined by western blot analysis. Thus, the LDLR is not degraded by PCSK9 on the cell surface. (BioMed Central)

    Nicotine: The Link Between Cigarette Smoking And Kidney Disease?  Jan 31, 2007
    Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7) ... Blots were incubated overnight with one of nine antibodies, which included mouse anti- 2-nAChR mAb s, anti- 3-nAChR mAb s, or anti- 4-nAChR mAb s. Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. (Science Daily)

    Nicotine: The link between cigarette smoking and kidney disease progression?  Jan 30, 2007
    Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7) ... Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. (EurekAlert!)

    Lack of Association between Antimyelin Antibodies and Progression to Multiple Sclerosis  Jan 25, 2007
    Antibodies were assessed by Western blot analysis at baseline, and the results compared with the time and rate of progression to clinically definite multiple sclerosis or a diagnosis of multiple sclerosis as defined by an international panel (the McDonald criteria) ... Conclusions Serum antibodies against MOG and MBP, as detected by Western blot analysis, are not associated with an increased risk of progression to clinically definite multiple sclerosis in patients who have had a clinically... (New England Journal of Medicine)

    Discovering biomarkers from gene expression data for predicting cancer subgroups usingneural networks and relational fuzzy clustering  Jan 6, 2007
    Our findings suggest the possibility of developing specialized microarray chips or use of real-time qPCR assays or antibody based methods such as ELISA and western blot analysis for an easy and low cost diagnosis of the subgroups. 1999-2007 BioMed Central Ltd unless otherwise stated. (BioMed Central)

    'A shocking lack of evidence'  Oct 26, 2006
    Both Montagnier and Colizzi have seen the results of a western blot, a test to detect proteins: they are "indeterminate", says Montagnier. "They say nothing," adds Colizzi. (Nature News Service)

    FDA CAPSULES  Oct 17, 2006
    NEW HIV TEST. The FDA has approved the Aptima HIV-1 RNA Qualitative Assay, the first test for detection of genetic material in HIV. The Aptima test can be used as an alternative to the Western blot, an existing test used to confirm positive results in HIV-screening tests. www. (Newsday -- Health)

    Nicotine Receptors On Kidneys May Speed Kidney Damage  Oct 11, 2006
    They found nicotine increased the production of fibronectin by about 50 percent as measured by western blot analysis, a laboratory procedure that detects proteins in a given sample of tissue, then measures protein levels with a specialized light meter. Cells with no nicotine had 36 relative light units (RLU), while cells exposed to nicotine had 51 RLU.. (Science Daily)

    FDA Approves Test To Help Diagnose Main Virus That Causes AIDS  Oct 6, 2006
    This test has important implications for medical diagnostic use because it could be a potential alternative to the traditional Western blot test now used for confirmation of HIV-1 infection when screening tests for HIV-1 antibodies are positive. In addition, the Western blot can, in some instances, be difficult to interpret and may not always provide a conclusive result. (U.S. Food and Drug Administration)

    An infectious agent of deception, exposed through proteomics  Sep 29, 2006
    A standard assay called Western blot confirmed the abundance increases of that small group of proteins during infection. . (EurekAlert!)

    CDC Recommends Broad HIV Screening  Sep 22, 2006
    If it is positive, a more specific test, called a western blot, is performed ... The lower figure is for a negative ELISA, the higher for a positive test that would require a western blot and more extensive post-test counseling. (The Ledger)

    Genome-wide gene expression profiling of human mast cells stimulated by IgE or Fc{epsilon}RI-aggregation reveals a complex network of genes involved in inflammatory responses  Aug 16, 2006
    Our data was validated using Real-Time-PCR; ELISA and western blot. We confirmed that IgE alone does not trigger mast cell-immediate responses, such as calcium signals, degranulation or protein-phosphorylation. (BioMed Central)

    Lyme disease: Facing Loudoun's silent epidemic  Aug 3, 2006
    "Western blot tests can produce false negatives," said Dr. Leila Zackrison, a Fairfax-based Lyme specialist and rheumatologist. "It still misses people; it's just a big mess.". (Centreville Times, VA)

    Form of palsy gains recognition  Jul 28, 2006
    Dear Dr. Donohue: Will you enlighten me on my husband's latest diagnosis -- progressive supranuclear palsy (Herald-Tribune)

    Slowing Alzheimer's disease by keeping mind and body active  Jul 24, 2006
    In accordance with this, a significant enhancement of microglial activity was found by Western blot and morphometric analyses of microglia, which often surround and infiltrate A; plaques. In addition, activating cellular protein degradation pathways provides another means of removing excess A. (EurekAlert!)

    Protein Tied To Usher Syndrome May Be Hearing's 'Missing Link'  Jun 28, 2006
    Additional experiments using western blot analysis, a technique that identifies individual proteins in a substance by separating them from one another by mass and testing how they react to certain antibodies, demonstrated that the antibody that recognizes protocadherin-15 in mice also binds to the TLA.. The team also analyzed the amino acid sequences of protocadherin-15 and discovered four distinct forms three of which are present in various developmental stages of the mouse inner ear. (Science Daily)

    The Netherlands reports second human case of mad cow disease  Jun 23, 2006
    A study published in 1996 indicated that a Western blot analysis of infecting prions obtained from 10 variant CJD patients and BSE-infected animals had similar molecular characteristics that were distinct from prions obtained from patients with other types of CJD (Nature 1996;383:685-90). An experimental study involving inoculation of a panel of inbred mice with the agents causing BSE and variant CJD substantially increased the strength of the scientific evidence for a causal association between... (Food Consumer)

    Long-term RNAi can be deadly  May 26, 2006
    Enzyme-linked immunosorbent assay (ELISA)-based screening and Western blot analysis found no evidence of. Another theory was that the shRNAs were toxic because they acted on non-target RNAs, "but we had similar lethal results with six different targets and 23 different shRNAs, making off-targeting unlikely as the cause," Kay said. (The Scientist)

    Sleuthing Lyme  May 1, 2006
    SF Chronicle Submissions. More than 25,000 people in the United States contracted Lyme disease in 2005, but the actual number of cases, the Centers for Disease Control and Prevention concedes, may be 10 times higher than that. (San Francisco Chronicle -- Opinion)

    Cloning and expression analysis of two distinct hypoxia-inducible factor alpha isoforms, gcHIF-1alpha and gcHIF-4alpha, from the hypoxia-tolerant grass carp  Apr 20, 2006
    Northern blot analysis showed that mRNA levels of gcHIF-1alpha and gcHIF-4alpha differ substantially under normoxic and hypoxic conditions, while Western blot studies demonstrated that the endogenous protein levels for both gcHIF-1alpha and gcHIF-4alpha are similarly responsive to hypoxia. Our findings suggest that both gcHIF-1alpha and gcHIF-4alpha are differentially regulated at the transcriptional and translational levels. (BioMed Central)

    Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties  Apr 11, 2006
    NF-kappa-B activation was measured by western blot and immunocytochemistry and cytokine secretion was detected by a protein array. Results: This study shows that S. pneumoniae isolates from pneumonia patients (n=298) can vary by as much as 1000-fold in their ability to bind to human lung epithelial cells. (BioMed Central)

    Alabama Cow With Mad Cow Born Before Feed Ban - USDA  Mar 20, 2006
    Officials confirmed mad cow in the animal on Monday, using the so-called Western blot test. A second confirmatory test that takes more time to complete, called the immunohistochemistry, also came back positive on Wednesday. (Planet Ark, United States)

    Electric stimulation enhances chemotherapeutic efficacy  Mar 18, 2006
    The relationship between MDR1 expression and the intracellular accumulation of doxorubicin as well as the cellular distribution of MDR1 was investigated by computerized image analysis immunohistochemistry and Western blot techniques ... Immunohistochemical, Western blot and fluorescence analysis revealed that AC not only decreases MDR1 expression but also changes its cellular distribution from the plasma membrane to the cytosol. (BioMed Central)

    USDAwill dig up infected cow  Mar 16, 2006
    First, two initial "rapid" screening tests were run, followed by a test called Western blot. The third, called immunohistochemistry, or IHC, was finished Wednesday. (MSNBC -- Health)

    R-CALF: Sample From Alabama Santa Gertrudis Positive For BSE  Mar 15, 2006
    The U.S. Department of Agriculture (USDA) on Monday announced that the National Veterinary Services Laboratory in Ames, Iowa, confirmed through a Western blot test that a Santa Gertrudis cow in Alabama tested positive for bovine spongiform encephalopathy (BSE). USDA officials said a dentition examination by a local veterinarian indicated the animal likely was at least 10 years old or more, meaning she was born before implementation of the 1997 feed ban. (CattleNetwork.com)

    Third U.S. BSE Case Won't Affect Talks on Beef Trade, Health Ministry Says  Mar 14, 2006
    The USDA laboratory in Ames, Iowa, confirmed the disease using a Western Blot test, John Clifford, the department's chief veterinary officer, said yesterday. Meat from the animal didn't enter the human or animal food chains, he said. (Bloomberg -- Japan)

    Mad Cow Disease Confirmed In Alabama  Mar 14, 2006
    Two versions of the initial "rapid" screening test suggested the cow had the disease, and a more detailed Western blot confirmed that finding ... Two versions of the initial "rapid" screening test suggested the cow had the disease, and a more detailed Western blot confirmed that finding. (CBS News)

    US Confirms 3rd Case of Mad Cow in 27 Months  Mar 14, 2006
    He said the new case was confirmed through the Western Blot test, which was added to USDA's battery of tests last year. But Clifford insisted that the discovery of a new case of mad cow disease in the United States should not scare other nations away from American beef. (Planet Ark, United States)

    Third US case of mad cow confirmed in Alabama cow  Mar 14, 2006
    A more accurate Western blot test at the National Veterinary Services Laboratory in Ames, Iowa, proved positive for BSE, said Dr. John Clifford, the U.S. Agriculture Department's top veterinarian. "The animal was buried on the farm and did not enter the animal or human food chains," Clifford told a teleconference with reporters. (Middle East North Africa Financial Network)


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